黄病毒变体在宿主范围,传染性和毒性方面有所不同
B S Congdon1, J R Baulch2, F F Filardo3
1Department of Primary Industries and Regional Development, 3 Baron-Hay Court, Kensington, Western Australia, 6151, Australia. benjamin.congdon@dpird.wa.gov.au.
Archives of virology
|August 10, 2023
概括
黄病毒 (TuYV) 变种表现出不同的表型,影响了甘的宿主范围和毒性. 了解这些差异对于管理这种经济上重要的植物病毒至关重要.
科学领域:
- 植物病毒学 植物病毒学
- 分子植物病理学 分子植物病理学
- 农业科学 农业科学
背景情况:
- 黄病毒 (TuYV) 是一种具有全球意义的,遗传多样性的病毒,影响各种作物,特别是甘 (Brassica napus).
- 澳大利亚TuYV分离体在开放式读取5 (ORF5) 中表现出显著的遗传变异,编码P5蛋白,这影响宿主适应和虫传播.
- 这种变异导致了三个不同的基因组:P5-I,P5-II和P5-III,具有有限的氨基酸序列相同性 (45-49%).
研究的目的:
- 调查代表P5-I,P5-II和P5-III变异的三个澳大利亚TuYV分离物之间的表型差异.
- 评估这些TuYV分离的宿主范围的变化,载体特异性,传播能力和毒性.
主要方法:
- 进行了温室实验,以评估三个TuYV分离物 (5414 (P5-I),5509 (P5-II) 和5594 (P5-III)).
- 主机范围测试了法巴豆,小豆和生菜.
- 使用Myzus persicae,Brevicoryne brassicae和Lipaphis pseudobrassicae进行了虫传播效率的评估.
- 用酶相关免疫吸收试验 (ELISA) 量化了卡诺拉中的病毒标位,并根据症状严重程度和种子产量影响评估了毒性.
主要成果:
- 隔离P5-I5414感染的法巴豆,P5-II5509感染的小,以及P5-I5414和P5-III5594感染的生菜.
- Myzus persicae传播了所有分离物,而B. brassicae和L. pseudobrassicae没有.
- 与P5-II5509和P5-III5594相比,P5-I5414显示了显著更高的传播率 (82%) 和卡诺拉病毒标位.
- P5-I5414在甘露中表现出更大的毒性,导致更严重的症状和产量损失.
结论:
- 在澳大利亚TuYV P5变种中存在表型变异,影响宿主范围,传播效率和毒性.
- 仅仅对ORF 5的分析不足以对TuYV菌株进行分类.
- 为了准确的菌株分类和有效的疾病管理策略,需要对现场分离物进行进一步的测序和表型.
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