改善微生物生产者和由此生产的食品安全控制方法
N R Efimochkina1, Yu M Markova2, Yu V Smotrina2
1Federal Research Centre of Nutrition, Biotechnology, and Food Safety, Moscow, Russia. efimochkina.natalya@yandex.ru.
Bulletin of experimental biology and medicine
|August 10, 2023
概括
这项研究强调了在食品生产中对转基因微生物 (GMM) 的分子遗传评估的必要性. 优化的DNA提取和测序方法改善了微生物生产者的鉴定和生物安全测试.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 食品科学 食品科学 食品科学
背景情况:
- 转基因微生物 (GMM) 在食品生产中越来越多地用于酶和生物活性物质.
- 在将它们纳入食品工业之前,评估转基因生物的生物安全性至关重要.
- 现有的检测转基因生物相关DNA的方法需要改进,以提高准确性和可靠性.
研究的目的:
- 为GMM生物安全建立一个分子遗传评估算法.
- 开发用于食品生产的转基因菌株测试的标准化方法.
- 为优化模具真菌的DNA提取和识别技术.
主要方法:
- 微生物生产者和食品对转基因转基因相关DNA (标记基因amp, lacZ) 的分析.
- 优化从模具真菌菌体中提取DNA,包括通过PCR进行净化和纯度评估.
- 桑格测序的适应用于模具真菌的识别和基因定型.
- 开发一种基因型定型算法,使用扩展原料和毛细血管电泳来对密切相关的微粒细胞进行定型.
主要成果:
- 在酶制剂和Aspergillus菌中发现了AMP和lacZ标记基因的高发病率.
- 一个优化的DNA提取程序提高了分子遗传试验的纯度和可靠性.
- 桑格测序成功识别了五种模两可的阿斯伯吉勒斯菌株.
- 开发了一种新的基因定型算法,用于精确识别Ascomycota micromycetes.
结论:
- 对于食品行业的转基因生物安全,需要一个强大的分子遗传评估算法.
- 标准化的GMM检测方法,特别是对于模具真菌,是必不可少的.
- 优化的DNA提取和先进的测序技术提高了转基因菌识别和生物安全评估的准确性.
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