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Updated: Jul 19, 2025

Zika Virus Specific Diagnostic Epitope Discovery

Zika Virus Specific Diagnostic Epitope Discovery

Published on: December 12, 2017

特定于寨卡病毒的中和抗体向二分体间包膜表位.

Rajeshwer S Sankhala¹, Vincent Dussupt², Gina Donofrio²

¹Emerging Infectious Disease Branch, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA; Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MD, USA.

|August 10, 2023

概括

研究人员确定了寨卡病毒 (ZIKV) 特定的抗体,可以中和病毒,而不会增强登革热病毒 (DENV) 疾病. 这些发现对于开发安全有效的ZIKV疫苗和治疗方法至关重要.

关键词:

CP: 免疫学免疫学在X射线晶体学.寨卡病毒寨卡病毒登革热病毒感染者弗拉维病毒 (flavivirus) 是一种病毒.一个单克隆抗体.中和抗体中和抗体.rhesus 子子子子子结构生物学结构生物学

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Last Updated: Jul 19, 2025

Zika Virus Specific Diagnostic Epitope Discovery

Zika Virus Specific Diagnostic Epitope Discovery

Published on: December 12, 2017

Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells

Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells

Published on: January 18, 2019

Evaluation of Zika Virus-specific T-cell Responses in Immunoprivileged Organs of Infected Ifnar1-/- Mice

Evaluation of Zika Virus-specific T-cell Responses in Immunoprivileged Organs of Infected Ifnar1-/- Mice

Published on: October 17, 2018

科学领域:

病毒学病毒学
免疫学免疫学免疫学
疫苗学疫苗学疫苗学

背景情况:

寨卡病毒 (ZIKV) 导致严重的先天性缺陷.
齐克病毒和登革热病毒 (DENV) 之间的交叉反应性使得疫苗开发复杂化,原因是潜在的抗体依赖增强.
识别ZIKV特异性抗体点对于安全的疫苗设计至关重要.

研究的目的:

为了分类ZIKV特异性抗体标.
开发针对ZIKV的中和单克隆抗体 (mAbs),这些抗体不会与DENV.交叉反应.
了解ZIKV的抗原风景,用于疫苗和治疗策略.

主要方法:

从感染ZIKV的 rhesus中分离了对ZIKV反应的B细胞和单克隆抗体 (mAbs).
将mAbs分组为不同的抗原组,针对ZIKV包膜糖蛋白.
对mAb-ZIKV包膜糖蛋白复合体的共同晶体结构的确定.
在康复期的人群中对抗体特异性的血清鉴定.

主要成果:

确定了ZIKV特异性mAbs的四个不同的抗原组,目标是在信封糖蛋白上的独特表位.
结构分析揭示了新的表位标,包括交叉原和二次二次相互作用.
所有四个确定的特异性都在从ZIKV感染中恢复过来的人类中发现.
来自所有群体的代表性mAbs在小鼠模型中赋予了对ZIKV的保护.

结论:

这项研究提供了对ZIKV特异性抗原性的关键见解,将其与DENV区分开来.
鉴定到的ZIKV特异性表位是开发下一代疫苗和治疗方法的有价值的目标.
这些发现支持开发安全的ZIKV疫苗,避免抗体依赖增强.