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Updated: Jul 19, 2025

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免疫光协议用于从各种模型和非模型哺乳动物的大脑组织中定位蛋白质标

Caterina Ciani1, Giulio Pistorio1, Marika Mearelli1

  • 1Falcone Lab, Department of Neuroscience, SISSA, Via Bonomea 265, 34123 Trieste, Italy.

STAR protocols
|August 10, 2023
PubMed
概括

这项研究提出了一种新的免疫光协议,用于哺乳动物大脑组织中的蛋白质定位. 该方法适用于新鲜和过度固定的样本,提高了研究可访问性.

关键词:
进化生物学 进化生物学神经科学是一个神经科学.

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Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
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