描述Proteus mirabilis和与之相关的等离子体,这些等离子体从无氧乳牛便消化器中分离出来
Gabhan Chalmers1, Rebecca E V Anderson1, Roger Murray2
1Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
PloS one
|August 10, 2023
概括
尿路感染的原因是Proteus mirabilis,它可以携带和转移扩展频谱类素 (ESC) 耐药基因和质粒. 这种机会性病原体可能是动物农业环境中抗菌素耐药性的被忽视的储存库.
科学领域:
- 微生物学 微生物学
- 抗微生物耐药性 抗微生物耐药性
- 环境健康 环境健康
背景情况:
- 奇迹性蛋白是一种机会性病原体,经常与人体感染有关,特别是尿路感染.
- 在动物衍生产品和便副产品中,P. mirabilis及其扩展频谱的抗素 (ESC) 菌株的流行程度仍然不太清楚.
- 了解P. mirabilis在抗菌素耐药性传播中的作用至关重要.
研究的目的:
- 从乳制品便无氧消化中分离的ESC耐药P.奇迹菌株的特征.
- 在农业环境中调查P. mirabilis中ESC耐药性的存在和遗传基础.
- 评估ESC耐药性水平基因转移的可能性.
主要方法:
- 从乳制品便无氧消化器中分离和识别P.mirabilis.
- 使用PCR进行特定β-乳糖酶基因 (blaCTX-M,blaCMY,blaSHV) 的查.
- 抗菌敏感性测试和耐药分离物的全基因组测序 (短读和长读).
- 结合实验用于评估等离子体转移效率.
主要成果:
- 在6个乳制品便消化器中,在4个中检测到ESC耐药的P. mirabilis.
- 在P. mirabilis分离物中确定了CTX-M-1和CTX-M-15耐药性等离子体.
- 全基因组测序提供了染色体和质粒的详细特征.
- 通过结合证明了ESC耐药性等离子体的高频转移到大肠杆菌.
结论:
- 奇迹性蛋白质 (Proteus mirabilis) 携带并可以有效地转移ESC耐药性基因和质粒.
- 乳制品便无氧消化器可以作为ESC耐药P.奇迹菌的储存库.
- P. mirabilis代表了一个潜在的重要,但却被忽视的,在农业环境中传播抗菌素耐药性的储存库.
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