在接受Helicobacter pylori第一线根除治疗的患者中,ESBL编码基因的丰富性和流行率
Dita Gudra1, Ivars Silamikelis1, Janis Pjalkovskis1
1Latvian Biomedical Research and Study Centre, Riga, Latvia.
PloS one
|August 10, 2023
概括
一个新的查小组有效地检测患者的扩展谱β-乳糖酶 (ESBL) 基因. 虽然ESBL基因的总体丰度仍然很低,但根除疗法在特定的ESBL基因患病率中显示出微小的变化.
科学领域:
- 微生物学 微生物学
- 遗传学 是一个遗传学.
- 临床医学 临床医学
背景情况:
- 扩展光谱β-乳糖酶 (ESBLs) 由于感染的治疗选择有限,造成了重大临床挑战.
- 产生ESBL的肠道细菌在医院和社区获得的感染中普遍存在.
- 了解ESBL基因的动态对于有效的抗菌药物管理至关重要.
研究的目的:
- 开发和评估一种新的查小组,用于检测广泛的ESBL编码基因.
- 评估ESBL基因在接受Helicobacter pylori根除治疗的患者中的丰度和流行率.
- 研究H. pylori根除治疗对肠道微生物群的功能概况,特别是ESBL基因分布的影响.
主要方法:
- 开发一个扩大面板,针对已知β-乳糖酶 (BL) 基因集群的保存区域.
- 使用150个便样本验证了该小组的效率,这些样本来自接受H. pylori根除治疗的患者.
- 下一代测序 (NGS) 和元基因组测序用于分析ESBL基因的库存和丰度.
主要成果:
- 开发的ESBL查小组有效地检测到大多数目标ESBL编码基因集群.
- 甲基因组分析显示ESBL基因的总体丰度非常低,在治疗状态之间没有显著的全球变化.
- 特定的ESBL基因集群,如blaOXY和blaADC,在根除后显著下降,而抗菌素耐药性 (AMR) 基因的总数增加.
结论:
- 开发的ESBL查小组是识别细菌DNA中ESBL编码基因集群的有效工具.
- 杆菌根除疗法导致ESBL基因流行率的轻微,集群特异性变化,同时增加了AMR基因的总体数量.
- ESBL基因分布在很大程度上是患者特异性的,这突显了需要个性化监测的必要性.
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