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隔离投影特异性和行为相关的杏仁体电路用于RNA测序

Yu-Ting He^1,2, Jia-Xin Zou^2,3, Ye He⁴

¹Queen Mary School, Nanchang University, Nanchang, China.

Current protocols

|August 10, 2023

概括

这项研究详细介绍了分离与焦虑类行为有关的特定神经元的方法. 这些技术使神经回路的综合分析和行为障碍的潜在治疗点成为可能.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
行为科学行为科学

背景情况:

环境因素显著影响神经电路活动和行为.
神经回路通过基因表达和改变细胞功能来适应环境变化.
了解这些适应机制对于剖析行为调节至关重要.

研究的目的:

描述标记和收集腹部海马投射的底侧杏仁体神经元的方法.
为了使行为相关的神经回路中转录水平的全面调查.
识别行为相关障碍的潜在致病和治疗点.

主要方法:

腺相关病毒注射用于神经电路标记.
急性脑切片隔离和光阳性细胞捕获.
cDNA图书馆建设用于转录组测序.

主要成果:

建立了隔离特定神经元群体的协议.
能够在目标神经回路内对基因表达进行详细的分析.
为发现行为背后的分子机制提供了基础.

结论:

描述的方法有助于研究调节行为的神经回路.
这种方法有助于识别治疗行为障碍的分子标.
综合转录组分析为神经适应和功能提供了洞察力.

关键词:

阿米格达拉的桃体一个循环的电路.神经提取神经提取转录转录是一种转录.腹部海马的一个海马体.

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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...