微机器人技术的双光子显微镜:在固定组织下方可视化微剂
Juan J Huaroto1, Luigi Capuano1, Mert Kaya1,2
1Surgical Robotics Laboratory, Department of Biomechanical Engineering, University of Twente, Enschede, The Netherlands.
PloS one
|August 10, 2023
概括
双光子显微镜能够更深入地可视化生物组织中的微剂,克服光学显微镜的局限性. 这一进步对于微机器人研究和潜在的体内应用至关重要.
科学领域:
- 生物医学工程 生物医学工程
- 光学成像技术的成像
- 机器人技术 机器人技术 机器人技术
背景情况:
- 光学显微镜提供高时空分辨率,但在生物组织中仅限于~100μm的透深度.
- 在组织表面下可视化微机器人剂 (微剂) 是当前微机器人研究的一个重大挑战.
- 双光子显微镜 (TPM) 提供更深的组织透 (>500微米) 和亚微米分辨率,在活组织成像和直接激光写作制造中具有应用.
研究的目的:
- 引入和评估二光子显微镜作为一种新的成像技术,用于在生物组织下方可视化微剂.
- 为了证明TPM在微机器人应用中对微剂的深层组织成像的能力.
- 探索TPM在推进体内微机器人可视化方面的潜力.
主要方法:
- 制造两种类型的微剂:外源性光剂染色的电纤维和自光树脂打印的生物灵感结构,使用直接激光写作 (DLW).
- 微剂的三维重建和激光与组织相互作用的定性研究的实验设置.
- 利用第二和生成,同时可视化微剂和周围组织.
主要成果:
- 证明了在甲固定组织下方的微剂的双光子显微镜成像,其最大透深度为800微米.
- 在135×135μm2视野内,以每秒1的速度实现磁性电纤维的连续成像.
- 成功地将微剂与组织结构一起可视化,证实了TPM的实用性.
结论:
- 双光子显微镜作为微机器人可行的替代成像方式,使微智能体的可视化超出了传统光学显微镜的局限性.
- 该研究在体外和外卵子条件下验证了微剂成像的TPM,为更深入的组织探索铺平了道路.
- 将TPM与微机器人集成具有未来体内应用的巨大潜力,增强微机器人系统在生物环境中的能力.
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