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Updated: Jul 19, 2025

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
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蛋白酶的结构指导工程,以改善其在寒冷条件下的活动

Fenghua Wang1, Xiangyang Ma1, Ying Sun1

  • 1Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, P. R. China.

Journal of agricultural and food chemistry
|August 10, 2023
PubMed
概括

研究人员设计了一种Bacillus蛋白酶,以增强其抗寒活性. 工程变体在10°C时显著提高了催化效率,为食品行业提供了潜在的应用,以保持食品质量和减少能源消耗.

关键词:
细菌蛋白质酶是一种细菌蛋白质酶.寒冷活性蛋白酶是一种冷活性蛋白酶.食品行业 食品行业 食品行业分子动力学分子动力学道工程是道工程的工程.

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Introduction to Mechanisms of Enzyme Catalysis01:13

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For many years, scientists thought that enzyme-substrate binding took place in a simple "lock-and-key" fashion. This model stated that the enzyme and substrate fit together perfectly in one instantaneous step. However, current research supports a more refined view scientists call induced fit. The induced-fit model expands upon the lock-and-key model by describing a more dynamic interaction between enzyme and substrate. As the enzyme and substrate come together, their interaction causes...
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