形成素抑制剂SMIFH2在山羊卵细胞成熟过程中抑制了细胞骨动力学和线粒体功能
Meng-Hao Pan1, Rui Xu1, Zhi Zheng1
1College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China; Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Yangling, Shaanxi, China.
Theriogenology
|August 10, 2023
概括
甲基因对山羊卵细胞成熟至关重要,维持细胞骨动力学和线粒体功能. 抑制形式素会导致由于反应性氧物种 (ROS) 的介质缺陷和亡.
科学领域:
- 生殖生物学 生殖生物学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 细胞骨对卵细胞中介成熟至关重要,影响核和细胞质发育.
- 素调节细胞骨组合,但它们在山羊卵细胞中的特定作用尚不清楚.
研究的目的:
- 为了研究形式素在山羊卵细胞中介成熟中的作用.
- 阐明胺抑制对卵子细胞骨组织,螺旋体完整性和线粒体功能的影响.
主要方法:
- 在山羊卵细胞中,甲胺活性被抑制.
- 评估了质进展,极体挤出和累积细胞扩张.
- 分析了动因动力学,螺旋/染色体结构和基因表达 (转录学).
- 量化了反应性氧物种 (ROS) 水平和亡.
主要成果:
- 胺抑制导致介质缺陷,包括阻碍极体挤出和异常的累积细胞扩张.
- 观察到改变的行为动态,受损的螺旋/染色体结构,以及被破坏的线粒体基因表达.
- 胺抑制诱导过度早期的亡,由活性氧物种 (ROS) 介导.
结论:
- 甲基因对维持细胞骨动力学至关重要,这对于山羊卵细胞中介成熟至关重要.
- 形式素在调节线粒体功能和预防卵细胞成熟期间ROS诱导的亡中发挥着重要作用.
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