相关实验视频
Updated: Jul 19, 2025

07:14
A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells
Published on: May 17, 2021
4.8K
缺氧优于人类ISCs,用于依赖interleukin的救援干细胞活动
Kristina R Rivera1, R Jarrett Bliton1, Joseph Burclaff1
1Joint Department of Biomedical Engineering, North Carolina State University and University of North Carolina at Chapel Hill, Raleigh, North Carolina.
Cellular and molecular gastroenterology and hepatology
|August 10, 2023
概括
缺氧使人肠干细胞 (hISCs) 处于休眠状态,但可逆,使它们对介质蛋白 (ILs) 产生反应并保持活动. 这揭示了炎症性缺氧期间肠道再生的机制.
科学领域:
- 胃肠病学 胃肠病学
- 干细胞生物学 干细胞生物学
- 缺氧研究 缺氧研究
背景情况:
- 由缺血或炎症引起的肠道缺氧会影响上皮质再生.
- 人体肠道干细胞 (hISCs) 对缺氧表现出耐受性,但它们的反应没有特征.
- 在缺氧下调查hISC行为对于了解肠道修复至关重要.
研究的目的:
- 研究长期缺氧对人类肠干细胞 (hISC) 功能的影响.
- 测试缺氧是否调节hISC对与炎症相关的白内素 (ILs) 的反应.
- 开发一种微生理学系统 (MPS) 用于在受控氧气水平下研究hISC.
主要方法:
- 在微生理系统 (MPS) 中,hISCs在低于1.0%的氧气下长达72小时培养.
- 评估了hISC活力,缺氧诱导因子1a (HIF1a) 反应,转录组学,细胞周期和细胞因子反应.
- 选HIF稳定剂和抑制剂以评估依赖HIF的途径.
主要成果:
- 在低氧状态下,hISCs在低氧状态下保持活力长达72小时,HIF1a在24小时达到峰值.
- 低氧诱导了hISCs的可逆休眠状态,改变了细胞周期和IL受体表达.
- 互白蛋白 (IL1β,IL2,IL4,IL6,IL10,IL13,IL25) 挽救了因低氧引起的hISC活动减少.
结论:
- 低氧会诱导hISCs的可逆休眠状态,增强它们对特定介质蛋白的反应能力.
- 这种原始化机制保留了hISC活动,为肠道上皮质再生提供了洞察力.
- 这些发现对于理解炎症性缺氧环境中的肠道修复至关重要.
相关概念视频
Regulation of Hematopoietic Stem Cells
3.2K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.2K
Multipotency of Hematopoietic Stem Cells
3.1K
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
3.1K
iPS Cell Differentiation
2.7K
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
2.7K
Chromatin Modification in iPS Cells
1.7K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.7K
Renewal of Intestinal Stem Cells
2.6K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
2.6K
Induced Pluripotent Stem Cells
4.2K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
Somatic...
4.2K

