在真菌细菌中,ATP独立的基质被招募到蛋白质酶体降解中
Tatjana von Rosen1, Monika Pepelnjak2, Jan-Philipp Quast2
1ETH Zurich, Institute of Molecular Biology and Biophysics, Zurich, Switzerland.
Life science alliance
|August 10, 2023
概括
细菌蛋白质组激活器 (Bpa) 针对热冲击抑制器HspR进行降解. 一个基板是一个基板.
科学领域:
- 细菌学 细菌学是一门学科.
- 分子生物学分子生物学
- 蛋白质降解 蛋白质降解
背景情况:
- 动态细菌利用蛋白质体的降解在恶劣的环境中生存.
- 细菌蛋白酶激活剂 (Bpa) 促进了热冲击抑制剂HspR.的能量独立降解.
- 由Bpa-蛋白酶组复合体选择和处理基质的机制尚未完全理解.
研究的目的:
- 阐明Bpa-蛋白酶组合体选择和加工基质的机制.
- 调查基质乱和特定领域在蛋白质体降解中的作用.
- 为了确定热冲击伴侣对基质稳定性和蛋白质体降解的影响.
主要方法:
- 生物化学试验用于研究基质结合和降解.
- 分析HspR,Bpa和DnaK之间的蛋白质与蛋白质相互作用.
- *Mycobacterium smegmatis*带有和没有Bpa.菌株的表型特征.
主要成果:
- 基质障碍对于Bpa介导的蛋白质体降解是必要的,但不够的.
- Bpa 绑定了 HspR 的 N-终端域,而 C-终端尾部则充当了线程处理手柄.
- 陪伴者DnaK稳定了HSPR,抑制了Bpa介导的降解.
- 在压力下对细菌的生存至关重要的是HspR的Bpa依赖性降解.
结论:
- Bpa-蛋白酶组复合体采用一种特定的机制,涉及基质域相互作用,以实现高效的降解.
- DnaK作为蛋白质酶体对HspR降解的负调节剂.
- 通过Bpa介导的HspR的蛋白质体降解对于菌根细菌的应激适应至关重要.
更多相关视频
09:57Examining Proteasome Assembly with Recombinant Archaeal Proteasomes and Nondenaturing PAGE: The Case for a Combined Approach
Published on: December 17, 2016
6.7K
09:57System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis
Published on: April 5, 2017
8.7K
相关概念视频
The Proteasome
892
Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
892
The Proteasome Structure
804
The ubiquitin-proteasome pathway is a well-known mechanism utilized by eukaryotic cells to remove cytoplasmic proteins that are misfolded, damaged, or no longer needed. In this pathway, the protein that needs to be eliminated undergoes a process called ubiquitination, where a chain of ubiquitin molecules is attached to the 48th lysine residue of the target protein. This ubiquitin modification helps the proteasome distinguish between a target protein and a healthy protein.
The proteasome is an...
The proteasome is an...
804
Translocation of Proteins into the Mitochondria
3.2K
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
3.2K
Regulated Protein Degradation
7.4K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
7.4K
Amino Acid Catabolism
51
Microorganisms rely on proteins as an essential carbon and energy source, particularly in environments with limited polysaccharides or lipids. However, proteins are too large to cross the plasma membrane unaided, necessitating enzymatic degradation. Microbes secrete extracellular proteases and peptidases that hydrolyze proteins into peptides, which can then be transported across the membrane. Once inside the cell, intracellular proteases degrade these peptides into free amino acids, which...
51
Destabilization of Microtubules
2.8K
The destabilization of microtubules can occur during different stages of the microtubule lifecycle, such as nucleation or elongation. It can take place at either end of the microtubule or in the microtubule lattices as a whole. The lifespan of individual microtubules within a cell varies according to the cell type and stage of the cell cycle. During interphase, the lifespan of the microtubule is about 30 minutes, while during cell division, it is about 15 minutes. In axonal microtubules of...
2.8K
