人类 exTreg细胞的鉴定为CD16+CD56+细胞毒性CD4+T细胞
Antoine Freuchet1, Payel Roy1, Sujit Silas Armstrong1
1La Jolla Institute for Immunology, La Jolla, CA, USA.
Nature immunology
|August 10, 2023
概括
调节性T (Treg) 细胞可以在动脉样硬化中变成exTreg细胞. 这些exTreg细胞被识别为炎症性,细胞毒性CD4+T细胞,与天然杀手细胞和Treg细胞不同.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 动脉样硬化研究 动脉样硬化研究
背景情况:
- 调节性T (Treg) 细胞在免疫恒温中发挥着至关重要的作用.
- 在动脉样硬化中,Treg细胞的一个子集可以分化成效应Treg (exTreg) 细胞.
- 了解exTreg细胞的独特特征对于了解动脉样硬化的免疫反应至关重要.
研究的目的:
- 在动脉样硬化背景下识别和描述exTreg细胞.
- 为了确定 exTreg 细胞的分子特征和功能性质.
- 将exTreg细胞与自然杀手 (NK) 细胞和常规Treg细胞进行比较.
主要方法:
- 利用可诱导的Treg和exTreg细胞谱系追踪器小鼠模型与易患动脉样硬化症的小鼠交叉.
- 采用大量RNA测序 (RNA-seq) 来分析被排序的小鼠Treg和exTreg细胞的转录组.
- 应用人类单细胞RNA测序 (scRNA-seq) 使用CITE-seq,流动细胞计,DNA测序和功能测试 (细胞杀死,CD107a降粒).
主要成果:
- 在老鼠和人类中保存的已识别的exTreg细胞特征基因 (CST7,NKG7,GZMA,PRF1,TBX21,CCL4).
- 人类的exTreg细胞的特征是CD3+CD4+CD16+CD56+细胞毒性CD4+T细胞.
- 在exTreg细胞中证明了Treg细胞序列的克隆扩张,并证实了细胞毒性功能.
- 显示的exTreg细胞与NK细胞和常规Treg细胞不同.
结论:
- 建立了 exTreg 细胞的分子和表型特征.
- 确定的exTreg细胞具有炎症和细胞毒性.
- 突出了在动脉样硬化中适应性免疫系统内的exTreg细胞的独特身份和功能.
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