永久土微生物对单细胞分化的影响In Vitro
S A Petrov1, Yu G Sukhovei1, L F Kalenova2
1Federal Research Center Tyumen Research Center, Siberian Division of the Russian Academy of Sciences, Tyumen, Russia.
Bulletin of experimental biology and medicine
|August 10, 2023
概括
从冷的Bacillus菌株中获得的微生物代谢物调节人类单细胞的分化. 温度依赖的代谢物影响单细胞亚群,表明免疫调节的潜力.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 生物化学 生物化学
背景情况:
- 微生物代谢物可以影响宿主免疫反应.
- 细菌物种以各种各样的生物活动而闻名.
- 单细胞分化成子群体在免疫中起着关键作用.
研究的目的:
- 为了研究来自化石Bacillus菌株的代谢物对人类单细胞分化的免疫调节作用.
- 为了确定代谢物生产温度是否影响它们对单细胞亚群的影响.
主要方法:
- 在体外培养人类外周血液单细胞7天.
- 用来自Bacillus sp.的代谢物进行治疗. 菌株2/09,9/08和Bacillus megaterium 8-75在不同的温度 (5°C,22°C,37°C) 中生产.
- 流细胞测量分析以评估单细胞亚种群变化 (经典,中间,非经典).
主要成果:
- 来自所有测试的Bacillus菌株和温度的代谢物减弱了古典单细胞 (CD14hiCD16-).
- 代谢物刺激了中间 (CD14+CD16+) 和非经典 (CD14loCD16+) 单细胞的分化.
- 8/75菌株的中温代谢物最有效地促进了中间单细胞分化,而8/75和2/09菌株的温暖代谢物增强了非经典单细胞分化.
结论:
- 来自特定细菌菌株的代谢物,受生产温度的影响,可以调节人类单细胞分化到不同的亚群.
- 巨杆菌菌株 8/75 和 巨杆菌 sp. 由于它们对中间和非经典单细胞的影响,2/09菌株代谢物显示出进一步研究的希望.
- 这些发现表明,在调节免疫反应方面,特别是在抗感染策略中,有潜在的应用.
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