CircLDLRAD3通过调节miR-558/Smad4/TGF-β的作用来抑制口腔状细胞癌的进展
Xue Zhang1,2, Guang-Yu Guo1,2, Ru-Yue Liu1,2
1The VIP Department, School and Hospital of Stomatology, China Medical University, Shenyang, China.
Journal of cellular and molecular medicine
|August 11, 2023
概括
在口腔状细胞癌 (OSCC) 中,循环RNA circLDLRAD3 的下调. 这种新型的循环RNA通过调节miR-558/Smad4/TGF-β轴来抑制OSCC的进展,提供潜在的治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 口腔状细胞癌 (OSCC) 呈现出显著的死亡率和发病率.
- 在OSCC中,分子机制,特别是涉及循环RNAs (circRNAs),尚未得到充分理解.
研究的目的:
- 研究一种新型圆形RNA,circLDLRAD3,在口腔状细胞癌中的作用和机制.
- 阐明了OSCC进展中涉及circLDLRAD3,miR-558和Smad4的监管网络.
主要方法:
- 定量PCR和光在现场杂交用于circLDLRAD3表达分析.
- 细胞增殖,迁移,入侵测定和体内动物实验,以评估circLDLRAD3的功能.
- 生物信息学,双露西法酶记者测试和RIP测试以确定分子相互作用.
主要成果:
- 在OSCC组织中,circLDLRAD3的表达显著下调.
- circLDLRAD3在体外和体内抑制OSCC细胞的增殖,迁移和入侵.
- circLDLRAD3 作为miR-558的海绵,对Smad4的表达进行上调,并影响TGF-β信号通路和上皮-介质细胞过渡 (EMT).
结论:
- circLDLRAD3通过抑制扩散和转移,在OSCC中起到瘤抑制作用.
- 圆DLRAD3/miR-558/Smad4/TGF-β轴代表了OSCC进展中的一个关键的监管途径.
- circLDLRAD3可能成为OSCC的潜在生物标志物和治疗点.
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