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相关概念视频

Resting Membrane Potential01:24

Resting Membrane Potential

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The relative difference in electrical charge, or voltage, between the inside and the outside of a cell membrane, is called the membrane potential. It is generated by differences in permeability of the membrane to various ions and the concentrations of these ions across the membrane.
The Inside of a Neuron is More Negative
The membrane potential of a cell can be measured by inserting a microelectrode into a cell and comparing the charge to a reference electrode in the extracellular fluid. The...
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Single-pass Transmembrane Proteins01:25

Single-pass Transmembrane Proteins

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Integral membrane proteins are tightly associated with the cell membrane and play a crucial role in cell communication, signaling, adhesion, and transport of the molecules. Some integral membrane proteins are present only in the membrane monolayer. For example, the enzyme fatty acid amide hydrolase is present in the cytoplasmic side of the membrane monolayer. In contrast, another type of integral membrane protein, also known as a transmembrane protein, spans across the membrane. Transmembrane...
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The Resting Membrane Potential01:21

The Resting Membrane Potential

133.0K
Overview
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Insertion of Multi-pass Transmembrane Proteins in the RER01:29

Insertion of Multi-pass Transmembrane Proteins in the RER

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The rough ER membrane synthesizes, assembles, and embeds transmembrane proteins in diverse topologies. These proteins function as transporters or channels and can remain in the ER membrane or are sent to the Golgi complex, lysosome, and cell membrane.
The multipass transmembrane proteins are the type IV integral membrane proteins with multiple topogenic sequences determining their spatial arrangement in the ER membrane. Nearly all multipass proteins lack a cleavable signal sequence and use...
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Insertion of Single-pass Transmembrane Proteins in the RER01:26

Insertion of Single-pass Transmembrane Proteins in the RER

6.8K
Integral membrane proteins are proteins adhered to the lipid bilayer of a cell organelle or membrane. They can be of two types: transmembrane integral proteins that span the lipid bilayer and monotopic proteins that are attached to either side of the membrane but do not pass through it.
Integral transmembrane proteins possess transmembrane and extra membrane domains. The transmembrane domains are primarily made of 20-25 hydrophobic amino acids arranged in a helical secondary confirmation. These...
6.8K
Mechanisms of Membrane Domain Formation00:59

Mechanisms of Membrane Domain Formation

3.0K
Different physical properties of lipids and proteins allow them to localize and form distinct islands or domains in the membrane. Some membrane domains are formed due to protein-protein interactions, whereas others are formed due to the presence of specific lipids such as sphingolipids and sterols—for example, large proteins, such as bacteriorhodopsin, aggregate and create distinct domains.
Another mechanism for membrane domain formation involves membrane proteins interacting with...
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相关实验视频

Updated: Jul 19, 2025

Measuring the Induced Membrane Voltage with Di-8-ANEPPS
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膜域反注册诱导一个内在的跨膜潜力.

Xiaoqian Lin1,2, Kaidong Lin1, Shiqi He1

  • 1Beijing Advanced Innovation Center for Biomedical Engineering, School of Engineering Medicine & School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China.

Langmuir : the ACS journal of surfaces and colloids
|August 11, 2023
PubMed
概括

膜域反注册,其中脂质和非对齐相反,产生局部膜不对称. 这种不对称性产生了内在的跨膜潜力,影响细胞功能.

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Transmembrane Domain Oligomerization Propensity determined by ToxR Assay
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Transmembrane Domain Oligomerization Propensity determined by ToxR Assay

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Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers
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Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers

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相关实验视频

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科学领域:

  • 细胞生物学 细胞生物学
  • 生物物理学的生物物理.
  • 分子动力学分子动力学

背景情况:

  • 等离子体膜形成纳米级域,如液体有序 (Lo) 脂质和液体无序 (Ld) 非.
  • 传单间的域动态,特别是反注册,仍然对生物影响的理解不佳.
  • 跨膜潜能对于细胞过程至关重要.

研究的目的:

  • 为了调查膜域反注册的生物相关性.
  • 探索反注册和跨膜潜力之间的关系.
  • 阐明胆固醇在这些现象中的作用.

主要方法:

  • 全原子分子动力学 (MD) 模拟.
  • 同焦点光显微镜实验.
  • 使用了HeLa和293T细胞系.

主要成果:

  • MD模拟表明与Lo/Ld膜反注册相关的内在的跨膜潜力.
  • 聚焦显微镜显示,胆固醇耗尽改变了细胞的跨膜潜力.
  • 实验结果与模拟结果一致,将胆固醇含量与潜在变化联系起来.

结论:

  • 膜域反注册会导致局部膜不对称.
  • 这种不对称性导致了内在的跨膜潜力.
  • 胆固醇在通过膜域组织调节跨膜潜能方面发挥着关键作用.