盐和表面活性剂对纳米粒子结构在纳米粒子和蛋白质二元系统中的竞争效应
Debasish Saha1,2, Sugam Kumar1,3, Jitendra P Mata4
1Solid State Physics Division, Bhabha Atomic Research Centre, Mumbai 400 085, India. sugam@barc.gov.in.
Physical chemistry chemical physics : PCCP
|August 11, 2023
概括
将盐和SDS表面活性剂添加到二氧化纳米颗粒蛋白溶液中会改变聚合. 盐会导致纳米粒子聚集,而SDS可以通过修改蛋白质相互作用,控制结构形成来防止它.
科学领域:
- 合体和表面科学科学
- 材料科学 材料科学 材料科学
- 生物物理学的生物物理.
背景情况:
- 纳米粒子和牛血清白蛋白 (BSA) 是阳离子的,通常保持分散.
- 蛋白质诱导的枯竭吸引力可以在更高度下引起纳米粒子聚合.
- 了解多组件系统对于控制纳米粒子组装至关重要.
研究的目的:
- 研究NaCl和SDS对纳米粒子-BSA系统结构的竞争效应.
- 使用对比匹配的小角度中子散射 (SANS) 探测纳米粒子结构.
- 阐明静电,疏水和枯竭相互作用在调整聚合物形态中的相互作用.
主要方法:
- 用于结构分析的小角度中子散射 (SANS).
- 动态光散射 (DLS) 用于研究相互作用和聚合.
- 使用对比匹配SANS专门观察纳米粒子.
主要成果:
- 盐添加触发了在临界盐度 (CSC) 的快速纳米粒子聚合,这是由于选的排斥和BSA诱导的枯竭吸引力,形成质量分数.
- 添加二甲基硫酸盐 (SDS) 通过通过BSA结合引入静电排斥来抑制CSC以下的聚合.
- 在CSC,SDS和BSA复合体上方,导致不可避免的集群,将聚合物从质量碎片转变为表面碎片.
结论:
- 这项研究证明了在多组件系统中对纳米粒子聚合结构的可调节控制.
- 相互作用是由盐度,蛋白质和表面活性剂的联合作用调节的.
- 这些发现为设计具有理想形态的复杂体系统提供了洞察力.
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