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多组学分析显示,布拉西卡特有的miR1885与菜种子对低温耐受性之间存在联系

Pengfei Xu^1,2,3, Wenting Zhang⁴, Xuan Wang⁵

¹Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.

Plant, cell & environment

|August 11, 2023

概括

研究人员确定miR1885是布拉西卡作物低温耐受性的关键调节剂. 调节miR1885水平可以增强大麻的抗寒能力,为改善作物繁殖提供了潜力.

科学领域:

植物科学植物科学
遗传学是一个遗传学.
分子生物学分子生物学

背景情况:

低温限制了黄铜 (Brassica) 作物生产.
布拉西卡 (Brassica) 寒冷耐受性的关键调节者在很大程度上是未知的.
了解这些调节器对于提高作物弹性至关重要.

研究的目的:

为了确定布拉西卡低温反应的转录后调节器.
研究微RNAs (miRNAs) 在寒冷耐受性中的作用.
探索miR1885在分子繁殖中的潜力.

主要方法:

小RNA测序和qRT-PCR用于在寒冷压力下分析miRNA表达.
全基因组关联研究 (GWAS) 以确定与耐寒性相关的候选miRNA基因.
5'快速放大cDNA末端 (5'RACE) 以验证miRNA目标.
在Brassica napus.中进行基因过度表达和淘汰实验.

主要成果:

16个已知的miRNAs对Brassica rapa的冷处理做出了反应,其中7个被证实.
在Brassica napus.中,GWAS确定了四个对寒冷有反应的miRNA基因,与低温抵抗位点相关.
miR1885与B. rapa和B. napus的低温反应有关.
miR1885的目标是Bn.TIR.A09和Bn.TNL.A03;它的过度表达降低了耐受性,而敲击则增加了耐受性.

结论:

miR1885在调节植物对布拉西卡低温反应方面发挥着关键作用.
miR1885及其向基因是培育耐低温布拉西卡作物的潜在候选者.
这项研究提供了对布拉西卡寒冷耐受性分子机制的见解.

关键词:

这就是Brassica Brassica.在GWAS中,GWAS就是GWAS.低温低温的低温是一个问题.miR18858585 在线观看在线观看小RNA测序的小RNA测序

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