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穿越他们的路径到外围涂抹:创伤红细胞的旅程

Chethana Mannem¹, Tuba Saher¹, Gayathri Bilagali Ramdas¹

¹Department of Pathology, Kempegowda Institute of Medical Sciences (Affiliated to Rajiv Gandhi University of Health Sciences), Bangalore, Karnataka, India.

Journal of laboratory physicians

|August 11, 2023

概括

血清细胞数量超过1%可靠地表明成年人患有血栓性微血管病性贫血. 坚持国际血液学标准化委员会指导方针可确保精确的手动血清细胞评估,并减少诊断变异性.

科学领域:

血液学血液学血液学
病理学病理学病理学
医学诊断医学诊断医学诊断

背景情况:

血栓性微血管病变 (TMA) 是一系列的疾病,而血栓性血栓细胞性紫外线 (TTP) 是一个危急的紧急情况.
晶体细胞是微血管损伤的关键指标,但也可以出现在非TMA条件中,如细胞骨异常,机械损伤和热损伤.
自动化石细胞计数方法缺乏可复制性,需要手动识别和标准化标准.

研究的目的:

使用国际血液学标准化委员会 (ICSH) 的指导方针,评估和量化TMA和非TMA组中的血栓性微血管性贫血细胞.
根据ICSH建议,评估手动石细胞计数的观察者间可重复性.

主要方法:

两名独立观察员使用ICSH光显微镜标准分析了157个带有分裂细胞的外周血液涂抹.
血液学发现与临床诊断和其他相关调查相关.
在最佳涂抹区域计数至少1000个红细胞后,以百分比进行了石细胞量化.

主要成果:

在5例TMA病例和152例非TMA病例中鉴定出分裂细胞.
与非TMA (0.76±0.67%) (p < 0.001) 相比,TMA (2.28±2.65%) 的平均晶状体百分比显著高于TMA (p < 0.001).
在应用ICSH指南时,高的观察者间相关系数 (0.59) 显示了对细胞计数的良好一致性.

结论:

超过1%的分裂细胞百分比是诊断成人血栓性微血管性贫血的强烈形态标记.
严格遵守ICSH手动胆固醇细胞计数指南,可以显著降低观察者间偏差,提高诊断准确度.

关键词:

在ICSH中.这种细胞是分裂细胞.血栓塞性紫外线的发生.这是一种血栓性血栓性疾病.血栓性微血管病变 - - 血栓性微血管病变

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