在小鼠中增加了activin A生物活性和丸体细胞瘤的精子命运
Penny A F Whiley1,2, Benedict Nathaniel1, Peter G Stanton1,2
1Centre for Reproductive Health, Hudson Institute of Medical Research, Clayton, VIC, Australia.
Frontiers in cell and developmental biology
|August 11, 2023
概括
在小鼠中,长期升高的激素A促进了精子干细胞 (SSCs) 通过丸 stromal 瘤的自我更新,但不是分化,提供支持的利基. 这一发现为改善SSC在体外传播提供了新的策略.
科学领域:
- 生殖生物学 生殖生物学
- 干细胞生物学 干细胞生物学
- 内分泌学 在内分泌学.
背景情况:
- 成年男性的生育能力依赖于精子干细胞 (SSCs),这些细胞根据微环境线索自我更新或分化.
- 活性蛋白A调节丸功能,其高水平与各种病理生理条件有关.
研究的目的:
- 调查慢性升高的活性蛋白A对丸微环境和精子命运的体内影响.
- 为了确定升高的activin A是否影响SSC自我更新与差异化.
主要方法:
- 检查了Inha淘汰赛 (KO) 小鼠的丸,这些小鼠的Activin A水平升高,并患有淋巴体 stromal 细胞瘤.
- 分析了GFRA1+ SSC群体,进行了瘤和管道组织的转录概况.
- 研究瘤细胞特征和相关的细胞透物.
主要成果:
- 在HA KO小鼠中,GFRA1+SSC的丰富性和扩散性增加,表明自我更新的增强.
- 在Inha KO小鼠中丸 stromal 瘤创造了一个支持SSC自我更新的微环境,富含GDNF,IGF1和FGF2等因素.
- 瘤细胞表现出改变的表型,瘤区域被各种细胞类型透,有助于SSC支持.
结论:
- 长期升高的活性蛋白A在体内会影响SSC的命运,通过促进一个有利于自我更新而不是差异化的利基.
- 在Inha KO小鼠中丸 stromal 瘤为了解SSC自我更新机制提供了一个模型.
- 这项研究提供了潜在的策略,以改善体外精子的繁殖.
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