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Updated: Jul 19, 2025

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通过从β-胺基标签中形成烯酸的小分子标记的简单净化

Kenji Hayamizu1, Kota Koike1,2, Kosuke Dodo1,2

  • 1Synthetic Organic Chemistry Laboratory, RIKEN Cluster for Pioneering Research 2-1 Hirosawa Wako Saitama 351-0198 Japan dodo@riken.jp sodeoka@riken.jp.

Chemical science
|August 11, 2023
PubMed
概括

这项研究从β-胺胺中引入一种稳定的乙烯酸,使一种新的净化系统成为可能. 这种方法有效地捕获和释放被标记的,使用水溶液中的β-胺胺亲和度标签.

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α-Alkylation of Ketones via Enolate Ions01:10

α-Alkylation of Ketones via Enolate Ions

Ketones with α protons are deprotonated by strong bases like lithium diisopropylamide (LDA) to form enolate ions. The anion is stabilized by resonance, and its hybrid structure exhibits negative charges on the carbonyl oxygen and the α carbon. This ambident nucleophile can attack an electrophile via two possible sites: the carbonyl oxygen, known as O-attack, or the α carbon, known as C-attack. The nucleophilic attack via the carbanionic site is preferred. This is due to the...
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