外部协调球对辅因子成熟和基质氧化受P460细胞染色体的影响
Melissa M Bollmeyer1, Sean H Majer1, Rachael E Coleman1
1Baker Laboratory Department of Chemistry and Chemical Biology Cornell University 162 Sciences Drive Ithaca NY 14853 USA kml236@cornell.edu.
在细胞染色体P460中封闭氨酸对酶成熟和氨酸氧化中的产品选择性至关重要. 这种残留物控制氧胺是否转化为氧化或氧化.
科学领域:
- 生物化学和酶学 生物化学和酶学
- 微生物的新陈代谢和循环.
背景情况:
- 用氨氧化细菌 (AOB) 氧化氨对于化至关重要.
- 像氧胺氧化还原酶 (HAO) 和细胞染色体P460 (cyt P460) 等金属酶在氧胺 (NH2OH) 氧化过程中控制产品选择性 (氧化与氧化).
- 细胞P460利用一种血红蛋白P460辅因子,结构研究表明一个封闭的氨残留物会影响其活性.
研究的目的:
- 调查Nitrosomonas europaea cyt P460.0.中的封闭氨酸残留物的作用.
- 确定该残留物如何影响NH2OH氧化过程中的辅因子成熟和产品选择性.
主要方法:
- 用于替代细胞P460.0中的封闭氨残留物,使用了局部导向的突变发生.
- 进行了酶活性测定,以评估在有氧条件下的NH2OH氧化.
- 进行了动态分析,以评估N2O形成和NO结合亲和力.
主要成果:
- 封闭氨酸的庞大的类侧链促进了对辅因子成熟至关重要的血红素-氨酸交叉链形成.
- 封闭氨酸残留物中的突变显著改变了产品的选择性,有利于N2O或NO的产生.
- 封闭氨酸侧链的固体和静电性质调节了N2O形成和NO结合的动力学.
结论:
- 细胞P460的外部协调球,特别是封闭氨酸,对于酶活性和产品特异性都至关重要.
- 这种残留物在促进自催化辅因子成熟和微调NH2OH的氧化途径方面发挥着双重作用.
- 了解这些机制可以了解AOB对循环的调节.
更多相关视频
04:32Extraction of Cofactor F420 for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples
Published on: October 14, 2021
09:33Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling
Published on: March 20, 2018
相关概念视频
Cofactors and Coenzymes
Cofactors can be metallic ions or organic molecules called coenzymes. These types of helper...
Electron Transport Chain: Complex III and IV
Introduction to Mechanisms of Enzyme Catalysis
The Electron Transport Chain
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
Factors Influencing the Rate of Chemical Reactions
Concentration and Pressure:
The more particles present within a given space, the more likely those particles are to bump into one another....
Role of Reduced Coenzymes NADH and FADH₂
