卡尔莫杜林6通过减弱番茄中的ICE1依赖性应激反应来负面调节耐寒性
Rui Lin1, Jianing Song1, Mingjia Tang1
1Department of Horticulture, Zijingang Campus, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, PR China.
Plant physiology
|August 11, 2023
概括
番茄CALMODULIN6 (CaM6) 通过与CBF表达1的诱导体 (ICE1) 相互作用来负面调节耐寒性,抑制耐寒基因. 这揭示了植物寒冷应激反应中的CaM6/ICE1模块.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 压力生理学 压力生理学
背景情况:
- 寒冷压力触发了细胞质 (Ca2+) 在植物中信号转导的增加.
- 卡尔莫杜林 (CaMs) 是Ca2+传感器,但它们在植物耐寒性中的作用尚未完全理解.
研究的目的:
- 描述番茄CALMODULIN6 (CaM6) 基因在应对寒冷压力的功能.
- 阐明CaM6影响番茄 (Solanum lycopersicum) 耐寒性的分子机制.
主要方法:
- 在冷刺激下对CaM6的基因表达分析.
- 番茄植物中CaM6的过度表达和沉默.
- 在CaM6和CBF表达1的诱导体 (ICE1) 之间进行了蛋白-蛋白相互作用研究.
- RNA测序 (RNA-seq) 和染色体免疫沉测序 (ChIP-seq) 用于确定下游目标.
主要成果:
- 寒冷会诱导CaM6的表达;CaM6的过度表达会增加对寒冷的敏感性,而沉默会导致对寒冷的不敏感性.
- CaM6与ICE1相互作用,独立于Ca2+,ICE1对于耐寒性至关重要.
- ICE1直接调节了76个冷反应 (COR) 基因.
- 与ICE1结合的CaM6减弱了ICE1的转录活性,抑制了ICE1依赖的COR基因表达.
结论:
- CaM6通过抑制 ICE1 介导的 COR 基因表达,对番茄的寒冷耐受性产生负面影响.
- 提出了一个CaM6/ICE1调节模块,其中ICE1在冷应力下对CaM6进行表达式作用.
- CaM6在调节ICE1活动方面发挥着作用,为植物寒冷应激机制提供了新的见解.
更多相关视频
相关概念视频
Responses to Heat and Cold Stress
13.6K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.6K
Responses to Salt Stress
13.2K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
13.2K
Calmodulin-dependent Signaling
5.2K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
5.2K
Regulation of Transpiration by Stomata
28.4K
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
28.4K
Adaptations that Reduce Water Loss
25.7K
Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
25.7K
Gene Regulation During Sporulation
34
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
34


