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微RNA-200a/b/c在中基底下丘脑核中的表达与衰老有关

Valentina V Porseva¹, Lydia G Pankrasheva², Konstantin Yu Moiseev²

¹Department of Pathophysiology, Yaroslavl State Medical University, ul. Revoliucionnaya 5, Yaroslavl, 150000, Russia.

MicroRNA (Shariqah, United Arab Emirates)

|August 11, 2023

概括

衰老会降低特定的下丘脑核中的microRNA (miRNA) 表达. 这项研究研究了雄性和雌性大鼠的miR-200a-3p,miR-200b-3p和miR-200c-3p水平,揭示了与年龄相关的下降和性别差异.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
老年学是指老年学的学科.

背景情况:

微RNA (miRNA) 是小型非编码RNA,在转录后调节基因表达.
下丘脑在平衡,适应和老化调节中起着至关重要的作用.
脑下垂体miRNA表达的变化可以显著影响衰老过程.

研究的目的:

为了研究miR-200a-3p,miR-200b-3p和miR-200c-3p的表达模式.
分析这些miRNA表达在特定的下丘脑核 (DMN,VMN,ARN).
为了检查与年龄相关的变化和性差异miRNA表达在老鼠.

主要方法:

使用定量实时PCR (qPCR-RT) 来测量miRNA的表达.
2 - ΔΔCq 算法用于数据规范化和分析.
评估表达水平在雄性和雌性老年老鼠的背中核 (DMN),腹中核 (VMN) 和弧形核 (RNA).

主要成果:

随着年龄的增长,观察到miR-200a-3p,miR-200b-3p和miR-200c-3p表达的总体下降.
针对这些miRNAs,男性的DMN和女性的VMN的特异性下降被注意到.
性别特定的变化包括VMN (男性) 和DMN (女性) 的miR-200b-3p降低,RNA (男性) 和DMN (女性) 的miR-200c-3p降低. 没有发现女性RNA的变化.

结论:

衰老与结核下垂体核中的miR-200a-3p,miR-200b-3p和miR-200c-3p的表达减少有关.
在老鼠中,这些与年龄相关的miRNA表达变化中发现了显著的性别差异.

关键词:

这是一个微型RNA.老化的老化衰老的老化在下丘脑中,下丘脑 qPCR-RT 是一个可靠的方法.一个老鼠,一个老鼠.结核核中的核.

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