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基于无标签的aptamer和金纳米酶的颜色测量感应器用于检测甲素A

Jidong Tang¹, Bing Tian¹, Xiaoqi Tao^2,3

¹College of Food Science, Southwest University, Chongqing, 400715, People's Republic of China.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry

|August 11, 2023

概括

一个新的口味传感器精确地检测到谷物中的 ochratoxin A (OTA). 这种无标签的方法使用黄金纳米粒子和体,用于食品安全中的敏感和可靠的真菌毒素分析.

科学领域:

分析化学分析化学
纳米技术纳米技术
食品安全食品安全

背景情况:

毒素A (OTA) 是一种有害的真菌毒素,经常在各种食品中发现.
准确和敏感的OTA检测方法对于确保食品安全和预防健康风险至关重要.
黄金纳米粒子 (AuNPs) 具有独特的特性,可以用于生物传感应用.

研究的目的:

开发一种新的无标签色度感应传感器,用于检测Ochratoxin A (OTA).
为了研究OTA体,黄金纳米粒子 (AuNPs) 之间的相互作用,以及它们对AuNPs的过氧化酶类活性的影响.
为了验证口味传感器在检测不同食品矩阵中的OTA方面的性能.

主要方法:

使用特定于OTA的aptamers来修改金纳米颗粒 (AuNPs).
研究了OTA结合对AuNP表面电荷密度和基基和Au3+释放的作用.
开发了一种基于AuNPs增强的过氧化酶类活性的无标签色度测试.

主要成果:

通过OTA与其胺体结合,AuNPs上的负电荷密度增加,促进基和Au3+的释放.
开发的口味传感器可以在10-600nm范围内实现精确的OTA检测.
对OTA.实现了6.20nM的低检测极限.
胃感应器在检测各种谷物 (如麦,玉米,大豆,大米和质大米) 中的OTA中表现出成功的应用.

结论:

该研究成功开发了一种用于OTA检测的无标签色度测量适应感应器.
吸食传感器具有很高的灵敏度和特异性,有可能在食品安全方面得到实际应用.
这些发现突出了阿普坦和AuNP在增强生物感知能力方面的协同效应.

关键词:

一个适应传感器.黄金纳米颗粒的使用方法奥克拉托克辛A是一种类似过氧酶的活性.

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