晚进化的鱼和鱼加入了GnRH1俱乐部
Kristian R von Schalburg1, Brent E Gowen2, Kris A Christensen3
1Department of Biology, Electron Microscopy Laboratory, University of Victoria, Victoria, BC, V8W 3N5, Canada. krvs@uvic.ca.
Histochemistry and cell biology
|August 11, 2023
概括
研究人员在大西洋鱼的性腺体中发现了淋巴激素释放激素1 (GnRH1),挑战了以前的信念. 这种激素在早期的生殖细胞发育和繁殖中起作用.
科学领域:
- 生殖生物学 生殖生物学
- 内分泌学 在内分泌学.
- 基因组学就是基因组学.
背景情况:
- 众所周知,三文鱼表达多种性腺激素释放激素 (GnRH) 形式.
- 以前的研究表明,像鱼和鱼这样的后期进化的鱼只具有两种GnRH类型 (GnRH2和GnRH3).
研究的目的:
- 调查大西洋鱼性腺体中GnRH1的存在和功能.
- 在鱼中分析GnRH1,GnRH2和GnRH3的遗传和表达模式.
主要方法:
- 快速放大cDNA末端 (RACE) 的方法
- 实时定量PCR (qPCR) 是一种实时定量PCR.
- 免疫组织化学 免疫组织化学
- 基因组组合分析的分析
主要成果:
- 证据证实了大西洋鱼性腺体中GnRH1的表达.
- 鱼基因组包含一个可行的GnRH1前的基因,尽管GnRH1同源对激素产生是非功能性的.
- 与GnRH2和GnRH3mRNAs的四个子结构相比,GnRH1mRNA具有明显的三个子结构.
- GnRH1的表达很广泛,在卵巢中表达的水平最高,而 GnRH2和 GnRH3主要表达在大脑中.
- 在早期的精子和卵细胞中免疫学检测到GnRH1,这表明它在生殖细胞发育中的作用.
结论:
- 与之前的假设相反,GnRH1存在于鱼性腺体中,并且可能具有功能.
- 在生殖腺中GnRH1的表达似乎仅限于早期发育阶段.
- 在早期发育过程中,GnRH1可能会调节生殖细胞中的线粒和/或介质过程.
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