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玻璃化胚胎与新鲜卵子中的胚胎:有没有区别?

Shelun Tsai¹, Jasmyn Johal², Jonas Malmsten²

¹Ronald O. Perelman and Claudia Cohen Center for Reproductive Medicine, Weill Cornell Medical College, 1305 York Avenue 6th Floor, New York, NY, 10021, USA. set9051@med.cornell.edu.

Journal of assisted reproduction and genetics

|August 11, 2023

概括

卵细胞玻璃化和加热用于植入前遗传测试对无细胞化 (PGT-A) 的基因测试显示了与新鲜卵细胞相比的胚胎化率. 冷保存不会对经过PGT-A的胚胎的染色体能力产生不利影响.

科学领域:

生殖医学生殖医学
遗传学遗传学是一个
胚胎学胚胎学

背景情况:

卵细胞冷保存是辅助生殖技术的关键组成部分.
预植入前遗传测试 (PGT-A) 评估胚胎的染色体状态.
对玻璃化和新鲜卵子之间的结果进行比较对于优化生育治疗至关重要.

研究的目的:

在接受PGT-A的患者中,使用玻璃化卵子与新鲜卵子对比胚胎育率.
评估卵细胞玻璃化和加热对染色体能力的影响.
为了评估PGT-A在卵子冷保存后的疗效.

主要方法:

一项回顾性队列研究比较了接受初次试管婴儿周期的患者用玻璃化卵子与使用新鲜卵子的年龄匹配对照.
所有患者都接受了对PGT-A.的皮层组织活检.
这项研究将117名具有玻璃化卵子细胞的患者与351名具有新鲜卵子细胞的对照进行了匹配.

主要成果:

在玻璃化和新鲜卵细胞组之间的euploidy (40.1%对41.6%),mosaicism (15.7%对12.0%),或aneuploidy (44.3%对46.4%) 的比率中没有观察到统计学上显著的差异.
在这两组患者中,每位患者获得的成熟卵细胞,受精卵细胞和胚芽细胞的数量相似.
患者的平均年龄为36.9岁,卵细胞玻璃化和变暖之间的中位数间隔为38个月.

结论:

卵细胞玻璃化之后进行升温,受精,并对PGT-A进行皮活检与对胚胎染色体能力的不良影响无关.
当通过PGT-A进行评估时,冷保存的卵细胞保持其产生染色体正常胚胎的潜力.
这些发现支持使用卵细胞玻璃化作为一种可行的生育保护和治疗选择.

关键词:

胚胎是平的胚胎是平的卵细胞的玻璃化.在植入前进行遗传检测.玻璃化的卵子细胞.

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