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在Arabidopsis thaliana中,miR397-LACs介导的应激耐受性

Shahid Ali^1,2, Shili Huang¹, Jiajie Zhou¹

¹Guangdong Provincial Key Laboratory for Plant Epigenetics; Shenzhen Key Laboratory of Marine Bioresource & Eco-Environmental Sciences, Longhua Institute of Innovative Biotechnology, College of Life Science and Oceanography, Shenzhen University, Shenzhen, 518060, Guangdong Province, China.

Plant molecular biology

|August 11, 2023

概括

沉默miR397通过增加叶绿素和素含量,减少氧化损伤,提高了阿拉比多普西斯的耐受性. 过度表达miR397会降低的耐受性.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
环境科学环境科学

背景情况:

(Cd) 是一种有毒的重金属,破坏了植物离子恒温.
微RNAs (miRNAs) 越来越多地被认为是植物应激反应中的作用.
了解miRNA介导的耐受机制对于农业应用至关重要.

研究的目的:

为了研究miR397在Arabidopsis thaliana中的耐受性中的作用.
阐明miR397影响积和植物反应的分子机制.
为了比较miR397过度表达与下调对耐受性的影响.

主要方法:

构建和分析miR397突变系 (过度表达:amiR397;下调:STTM miR397).
测量叶绿素,胡卜素和红素含量.
感应合等离子体 - 光学发射光谱仪 (ICP-OES) 用于量化.
对活性氧物种 (ROS) 和抗氧化酶活性 (CAT,MDA) 的测定.
对传送基因表达的分析.

主要成果:

下调的miR397 (STTM miR397) 菌株表现出增强的耐受性,含有更高的叶绿素,胡卜素和素含量.
与野生类型和amiR397.397相比,STTM miR397植物的细胞壁含量增加和总含量降低.
沉默miR397降低了活性氧物种 (ROS) 和抗氧化酶活性,表明氧化损伤减少.
过度表达miR397 (amiR397) 导致转运基因的表达更高,耐受性降低.

结论:

miR397在Arabidopsis thaliana中调节耐受性方面发挥着重要作用.
下调miR397通过调节细胞壁组成和减少氧化应激来提高耐受性.
过度表达miR397会对耐受性产生负面影响,可能是通过调节输送基因和素生物合成.

关键词:

抗氧化剂是一种抗氧化剂.在Cd压力压力.这就是LACs基因.在Lignin的基础上.这就是miRNAs.

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