采样系统的采样类型,频率和规模对SARS-CoV-2量化准确性的影响
Andrea D George1,2, Devrim Kaya2, Blythe A Layton1
1Department of Research & Innovation, Clean Water Services, Hillsboro, Oregon 97123, United States.
概括
废水的复合采样更可靠地检测SARS-CoV-2,特别是在较小的排水区. 采集样本经常错过检测,并且与不同水域规模的复合样本相比,显示出更高的变异性.
科学领域:
- 环境科学环境科学
- 公共卫生 公共卫生
- 病毒学 病毒学
背景情况:
- 基于废水的流行病学 (WBE) 对于监测COVID-19等传染病至关重要.
- 快速实施SARS-CoV-2的WBE采样可能忽略了采集区特定的因素.
- 选择采样方法 (取与复合) 可以显著影响WBE数据.
研究的目的:
- 评估抓取与复合采样对SARS-CoV-2检测和量化的影响.
- 评估不同的排水流域规模如何影响这些采样方法的性能.
- 确定废水中SARS-CoV-2监测的最佳采样策略.
主要方法:
- 对SARS-CoV-2的抓取和复合采样策略的比较.
- 在四个不同的流域尺度上进行分析:高流量 (WWTP影响),中流量 (社区),低流量 (城市街区) 和超低流量 (建筑).
- 对SARS-CoV-2RNA度的量化和检测率的评估.
主要成果:
- 在高流量地点,采集样本与24小时复合样本相当,所有样本中检测到SARS-CoV-2.
- 随着水域规模的减少,采集样本显示了负面结果的百分比增加,与复合材料相比,度差异高达2log10单位.
- 复合采样在捕获真实SARS-CoV-2度方面表现出更高的准确性,特别是在超低流量地点.
结论:
- 复合采样优于抓取采样,用于在废水中检测SARS-CoV-2,特别是在较小的水域.
- 复合采样有效地弥补病毒信号的时间变化,与抓取采样不同.
- 优化采样策略对于准确监测公共卫生威胁的WBE至关重要.
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