同时的RNA和DNA吸收学使用单个数据独立获取质谱分析分析
Giulia Martella1, Nisha H Motwani2, Zareen Khan1
1Department of Environmental Science, Stockholm University, Stockholm SE-106 91, Sweden.
Chemical research in toxicology
|August 11, 2023
概括
研究人员开发了一种新的方法,使用高分辨率质谱仪在生物体中同时检测RNA和DNA附加物. 这种补充学方法在波罗的海两足动物中发现了新的RNA补充物,可能作为污染影响的生物标志物.
科学领域:
- 环境化学环境化学
- 分子生物学分子生物学
- 分析化学是一种分析化学.
背景情况:
- 吸收学研究分析核酸和蛋白质的化学修饰.
- 高分辨率双重质谱 (HRMS/MS) 已经推进了添加学方法.
- 同时分析RNA和DNA添加物具有挑战性,但有价值.
研究的目的:
- 开发和演示一种基于HRMS的新方法,用于同时进行RNA和DNA adduct分析.
- 将这种方法应用于波罗的海两足动物,作为环境干扰的哨兵.
- 在这些生物体中识别和表征RNA附加物.
主要方法:
- 开发了一种使用Orbitrap HRMS的单注射HRMS方法,具有全扫描和数据独立获取.
- 利用开源程序"nLossFinder"根据特征中性损失进行引证识别.
- 使用MODOMICS数据库进行RNA添加物的结构识别.
主要成果:
- 在波罗的海两足动物中成功检测到大约60个假定RNA附加物,除了已知的DNA附加物外.
- 鉴定到的RNA添加物包括甲基化和去胺化产物 (伊诺辛).
- 54个RNA添加物在结构上仍然未被识别,这表明可能有新的发现.
结论:
- 开发的多附带学方法使得RNA和DNA附带物的同时选成为可能.
- 这种方法在环境哨兵中确定了新型RNA添加物.
- 该方法具有开发生物标志物的潜力,用于诊断生物界污染影响.
更多相关视频
12:15Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
Published on: May 29, 2019
8.8K
10:52Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
Published on: September 28, 2017
8.2K
相关概念视频
03:21RNA-seq
10.1K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.1K
01:33Peptide Identification Using Tandem Mass Spectrometry
6.5K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.5K
01:21Mass Spectrometry: Complex Analysis
825
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
825