从Campylobacter jejuni分泌的一种Sirtuin在感染期间有助于中性粒细胞激活和肠道炎症
Sean M Callahan1, Trevor J Hancock1,2, Ryan S Doster3,4
1Department of Microbiology, University of Tennessee, Knoxville, TN 37996, USA.
Science advances
|August 11, 2023
概括
坎比洛巴克特 (Campylobacter jejuni) 分泌一种Sirtuin,通过 deacetylating histones来激活人类中性粒细胞,从而在感染期间引起炎症和组织损伤. 这种细菌的sirtuin是一种新的促进疾病的效应因子.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 基质子的修饰调节了真核细胞的过程,包括炎症.
- 细菌病原体可以操纵宿主因素,如sirtuins促进感染.
研究的目的:
- 为了识别和描述来自Campylobacter jejuni.的细菌sirtuin.
- 为了研究这种sirtuin在宿主中性粒细胞激活和campylobacteriosis病变发生中的作用.
主要方法:
- 来自Campylobacter jejuni.的一种脱乙酶的鉴定和生物化学表征.
- 对人类中性粒细胞进行体外研究,以评估Sirtuin活性和基因素修饰.
- 在体内研究中,使用了带有细菌sirtuin突变的野杆菌病的小鼠模型.
主要成果:
- 确定了一种具有Sirtuin活性的Campylobacter jejuni脱乙酶,并表明它被分泌到宿主中性粒细胞中.
- 细菌的sirtuin去乙基化宿主组织蛋白,促进中性粒细胞的激活和细胞外陷的产生.
- 缺少这种sirtuin的突变体殖民了肠道,但未能在小鼠中诱导炎症,细胞因子产生或组织病理.
结论:
- 分泌的细菌sirtuins代表了一种新的细菌效应剂类.
- 细菌通过分泌的Sirtuins对宿主组织组织蛋白进行修饰,导致campylobacteriosis的炎症和病理.
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