相关实验视频

Updated: Jul 19, 2025

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

MS Annika 2.0 在高灵敏度和高特异性的MS2-MS3-基于工作流中识别交叉链接

Micha J Birklbauer¹, Manuel Matzinger², Fränze Müller²

¹Bioinformatics Research Group, University of Applied Sciences Upper Austria, Softwarepark 11, 4232 Hagenberg, Austria.

Journal of proteome research

|August 11, 2023

概括

MS Annika 2.0通过识别更多真正的蛋白质-蛋白质相互作用来增强交联质谱. 这个新版本提高了精度,并减少了复杂的MS3光谱分析中的假阳性.

科学领域:

蛋白质组学是指蛋白质组学.
结构生物学结构生物学
生物化学生物化学

背景情况:

交联质谱 (MS) 对于识别蛋白质-蛋白质相互作用和阐明蛋白质结构至关重要.
此前开发的搜索引擎MS Annika使用MS2光谱准确识别交联.
在MS技术的进步需要更新的计算工具进行全面的分析.

研究的目的:

推出MS Annika 2.0,一个增强的交叉链接搜索引擎.
实施一种新的搜索算法,支持基于MS2-MS3的类鉴定方法.
为在多个多发性硬化症阶段中识别的类引入新的评分功能.

主要方法:

MS Annika 2.0 开发了新的搜索算法和对MS2-MS3数据的评分功能.
该软件的MS3搜索功能在五个不同的数据集上进行了评估.
性能与现有的交叉链接搜索引擎XlinkX和MaXLinker.com进行了比较.

主要成果:

MS Annika 2.0在识别MS3光谱中的交联上表现出卓越的性能.
与其他工具相比,更新的引擎检测到多达四倍的独特交叉链接.
安妮卡2.0实现了更准确的错误发现率 (FDR) 估计,错误阳性较少.

结论:

MS Annika 2.0显著提高了交叉链接质谱分析的准确性和深度.
该软件提供了一种更可靠的方法来识别蛋白质-蛋白质相互作用和结构洞察力.
这些发现凸显了MS Annika 2.0在复杂蛋白质组学研究中的实用性.

关键词:

在MS3中,MS3是MS3,MS3是MS3.这是一个PPI PPI.在XL-MS中使用XL-MS 生物信息学是一种生物信息学.交叉链接是指交叉链接.蛋白质与蛋白质的相互作用搜索引擎搜索引擎搜索引擎是什么

更多相关视频

A Hydrogen-Deuterium Exchange Mass Spectrometry HDX-MS Platform for Investigating Peptide Biosynthetic Enzymes

A Hydrogen-Deuterium Exchange Mass Spectrometry HDX-MS Platform for Investigating Peptide Biosynthetic Enzymes

Published on: May 4, 2020

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

Published on: October 15, 2019

相关概念视频

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

Tandem Mass Spectrometry

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and signal-to-noise ratio for the analyte. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.
Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called collision-induced...