来自Antrodia cinnamomea的Cubebol-生产的塞斯基烯合成酶的表征和晶体结构
Sheng-Chia Chen1,2, Bo-Chen Jiang1, Yen-Ju Lu3
1Department of Agricultural Chemistry, National Taiwan University, Taipei 10617, Taiwan.
Journal of agricultural and food chemistry
|August 11, 2023
概括
研究人员特征了来自Antrodia cinnamomea的甲合成酶AcCop4. 这种酶产生cubebol,一种有价值的食品添加剂,为其生物合成途径和潜在的工业应用提供了洞察力.
科学领域:
- 生物化学 生物化学
- 酶学 是一种酶学.
- 自然产品 化学 化学
背景情况:
- 台湾特有菌类Antrodia cinnamomea,其药用特性归因于其类化合物.
- 由于其生物活性,来自A. cinnamomea的类是潜在的食品添加剂.
- 了解这些化合物的生物合成途径对于它们的工业利用至关重要.
研究的目的:
- 从功能和结构上描述来自Antrodia cinnamomea的基合成酶AcCop4.
- 通过AcCop4.4阐明由Cubebol生产的生物合成机制.
- 为进一步生产和修改立方体提供结构基础.
主要方法:
- 对AcCop的功能性特征4.
- 酵素产品的气色谱-质谱学 (GC-MS) 分析.
- 用酸盐和离子复合的AcCop4的X射线晶体学.
主要成果:
- AcCop4催化了法纳西尔酸盐 (FPP) 的循环化,主要产生立方体.
- AcCop4的晶体结构显示了R311.1.介导的活性部位的关闭.
- 聚酸和离子的结合并没有诱导G1/2螺旋体的显著构造变化,这表明部分封闭的apo形式.
结论:
- 该研究提供了AcCop4的高分辨率结构,有助于理解其生物合成机制.
- 这些发现支持了增加生产和修改食品工业应用中的cubebol的潜力.
- AcCop4是cubebol生物合成中的关键酶,对食品添加剂开发有影响.
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