固有耐药性粉类型3类型的类型是通过聚合成年便无菌体在体外发酵来决定的
C E Klostermann1, M F Endika2, E Ten Cate1
1Biobased Chemistry and Technology, Wageningen University & Research, Bornse Weilanden 9, 6708 WG Wageningen, the Netherlands; Laboratory of Food Chemistry, Wageningen University & Research, Bornse Weilanden 9, 6708 WG Wageningen, the Netherlands.
Carbohydrate polymers
|August 11, 2023
概括
耐性粉3型 (RS-3) 发酵产生有益的丁酸盐. RS-3的微观结构,特别是α-1,4葡萄糖的分子量分布,显著影响着酸盐的产生和肠道微生物群的组成.
科学领域:
- 微生物学 微生物学
- 营养科学 营养科学
- 生物化学 生物化学
背景情况:
- 肠道微生物群的耐性粉 (RS) 发酵产生有益于健康的丁酸盐.
- 耐性粉3型 (RS-3) 是一个重要的丁酸盐来源.
- 了解RS-3的物理化学特性对于优化丁酸盐生产至关重要.
研究的目的:
- 调查RS-3的物理化学特征如何影响发酵期间的酸盐生产.
- 确定RS-3链长度,分子量分布和晶体类型对肠道微生物群组成的影响.
- 为了确定最大限度地提高酸盐产量的特定RS-3特征.
主要方法:
- 在体外发酵六个内在RS-3基质与聚合成年便注射剂.
- 对链长度 (DPn),分子量分布 (PI) 和晶体类型 (A/B) 的RS-3基质的分析.
- 使用16S rRNA基因测序评估发酵后的微生物群组成.
主要成果:
- 所有固有的RS-3基板都产生了高水平的丁酸盐 (乙酸盐/丁酸盐比率≤2.5).
- 来自多分散性α-1,4葡萄糖的A型RS-3产生了最高的相对丁酸盐 (乙酸/丁酸盐比:1).
- 在RS-3发酵过程中,主要刺激了Lachnospiraceae,Bifidobacterium和Ruminococcus,其丰度因RS-3特征而有所不同.
结论:
- 由α-1,4葡萄糖分子量分布影响的RS-3的微观结构是丁酸盐生产的关键决定因素.
- 特定的RS-3物理化学特性调节了肠道微生物群的组成.
- 优化RS-3特性,特别是分子重量分布,可以增强有益的酸盐生产.
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