在小麦1BL.1RS转位中,1RS臂之间的不同亚端体结构影响了它们的介质重组,并诱导了它们的结构变化
Ziying Xiong1,2, Jie Luo1,2, Yang Zou1,2
1State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, China.
在小麦的1RS臂上,亚端粒合重复的变化会影响介质重组率. 类似的重复组合增强了重组,影响了1BL.1RS转位染色体上的基因定位.
科学领域:
- 植物遗传学 植物遗传学
- 分子生物学分子生物学
- 细胞遗传学 细胞遗传学
背景情况:
- 小麦-麦1BL.1RS转位在1RS臂上具有子端粒串联重复的特征.
- 了解重复组合对1RS介质重组的影响对于增强1BL.1RS转位染色体的遗传多样性至关重要.
研究的目的:
- 为了研究子端粒串联重复组合对1RS臂的介质性重组的影响.
- 开发一种可视化工具,用于检测1RS手臂中的介质重组.
主要方法:
- 使用了五种小麦-瑞1BL.1RS转移品种/交叉组合的线条.
- 使用寡核酸 (Oligo) 探针 (Oligo-s120.3,Oligo-TR72,Oligo-119.2-2,Oligo-pSc200,Oligo-pSc250) 来分析1RS臂上的信号模式.
- 在后代中量化重组率和识别的重组事件.
主要成果:
- 在不同小麦-麦线之间的探测器Oligo-s120.3,Oligo-TR72和Oligo-119.2-2之间确定了不同的信号模式.
- 观察到Oligo-pSc200信号强度和跨线存在的变化.
- 发现1组与2组相比,重组率明显较低,在1组后代中发现了不平等的重组和反转.
结论:
- 该研究提供了一种可视化方法来检测1RS臂中介性重组.
- 在pSc200串联重复的变化显著影响1RS臂介质重组率.
- 亚端粒重复组合影响1RS重组,从而通过介质重组影响基因局部化.
更多相关视频
09:40Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae
Published on: September 23, 2011
07:55Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
Published on: September 11, 2022
相关概念视频
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...
Crossing Over
Meiosis I
Prophase I is the most extended and complex step of meiosis I characterized by synapsis, chromosome pairing, and recombination of the homologous chromosomes. This process is facilitated by a proteinaceous structure called the...
Gene Conversion
Chromosome Structure
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
Overview of Transposition and Recombination
