鱼类凝的结构和功能性质通过用银-寡糖糖的糖化来改变
Yong Wang1, Caiyun Wu1, Hui Jia2
1State Key Laboratory of Marine Food Processing and Safety Control, National Engineering Research Center for Seafood, Collaborative Innovation Center of Provincial and Ministerial Co-Construction for Seafood Deep Processing, Liaoning Province Collaborative Innovation Center for Marine Food Deep Processing, College of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.
Foods (Basel, Switzerland)
|August 12, 2023
概括
galakto-oligosaccharide (GOS) 糖化增强了鱼类凝 (FG) 的界面特性,如乳液和泡稳定性. 适度的GOS治疗改善了FG功能,尽管过度修改减少了这些好处.
科学领域:
- 食品科学 食品科学 食品科学
- 生物化学 生物化学
- 蛋白质化学 蛋白质化学
背景情况:
- 鱼类凝 (FG) 是一种有价值的生物聚合物,具有多种应用.
- 修改FG的特性可以扩大其在食品和生物医学行业的实用性.
- 糖基化是一种潜在的方法来改变蛋白质的结构和功能.
研究的目的:
- 为了研究银-寡糖 (GOS) 糖化对鱼类凝 (FG) 的结构和功能特征的影响.
- 确定最佳的糖化条件,以改善FG的界面特性.
主要方法:
- 鱼类凝被用GOS糖化,持续时间不同.
- 使用SDS-PAGE,光谱和FTIR分析结构变化.
- 评估了功能性质,包括乳液和泡特性.
- 测量了凝强度,以评估对凝的影响.
主要成果:
- 增加的糖化化时间导致了更高的移植度和色.
- SDS-PAGE揭示了新的,更高分子量蛋白质带.
- 糖化降低了内在光,增加了表面的疏水性.
- FTIR分析表明α-螺旋的含量减少,随机卷轴的增加.
- 经过中度GOS治疗 (GOS-4,GOS-8),乳液和泡的稳定性显著改善.
- 延长的糖化 (超过12小时) 导致改善减弱,凝强度降低.
结论:
- 适度的银-寡糖 (GOS) 糖化有效地增强了鱼类凝 (FG) 的界面和乳化特性.
- 过度糖化对FG的功能属性和凝强度产生负面影响.
- 这项研究表明,受控的甘化作为功能化鱼类凝的可行策略.
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