基于单面碳纳米角的光能量共振转移aptasensor平台用于检测阿弗拉托克辛B1的
Yiting Fan1,2, Huanhuan Yang3,4, Jiaxin Li2,5
1College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China.
Foods (Basel, Switzerland)
|August 12, 2023
概括
一个新的基于aptamer的传感器可以快速且负担得起地检测出Aflatoxin B1 (AFB1). 这种方法使用aptamers和碳纳米角来检测食物中的敏感AFB1,为ELISA提供了切实可行的替代方案.
科学领域:
- 分析化学 分析化学
- 生物感应是一种生物感应.
- 食品安全 食品安全
背景情况:
- 非洲毒素B1 (AFB1) 对全球的健康和经济构成重大风险.
- 目前的AFB1检测方法往往是缓慢的,昂贵的,或需要专门的设备.
- 迫切需要快速,现场和经济的AFB1检测解决方案.
研究的目的:
- 开发一种新的适应感应平台,用于敏感和选择性检测阿弗拉托克辛B1.
- 为了利用单壁碳纳米角 (SWCNHs) 的光灭特性来检测AFB1.
- 建立一个实用且具有成本效益的现场AFB1分析方法.
主要方法:
- 制造一个AFB1体 (AFB1-Apt) 传感平台,采用光标记的体 (FAM).
- 利用SWCNHs的吸附灭功能来监测AFB1的存在.
- 机制:AFB1与AFB1-Apt的结合防止SWCNH吸附和光火,产生一个信号.
主要成果:
- 传感平台对AFB1.1具有很高的选择性.
- 在10-100 ng/mL的范围内观察到良好的线性反应.
- 实现了4.1 ng/mL的低检测极限 (LOD).
- 通过大豆油的增实验和与ELISA的比较成功验证.
结论:
- 开发的口味传感器为AFB1检测提供了快速,选择性和敏感的方法.
- 该平台为现场监测食品安全提供了一个有前途的工具.
- 该战略实用且具有成本效益,解决了改善AFB1分析的需求.
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