通过与六种黄类素的共价相互作用,降低β-乳糖球蛋白的抗原性和提高抗氧化能力
Pei Pu1, Zhifen Deng1, Lang Chen1
1Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400044, China.
Foods (Basel, Switzerland)
|August 12, 2023
概括
与天然黄酸盐共同修改β-乳糖球蛋白 (β-LG) 显著降低其抗原性,并增强其抗氧化特性. 这种修饰改变了蛋白质结构,为改善β-LG应用提供了一个有希望的方法.
科学领域:
- 食品科学 食品科学 食品科学
- 蛋白质化学 蛋白质化学
- 生物化学 生物化学
背景情况:
- β-乳糖球蛋白 (β-LG) 是一种具有功能性质的关键营养蛋白.
- 它的使用受到高抗原性和氧化易感性的限制.
- 制定改善β-LG安全性和稳定的策略至关重要.
研究的目的:
- 为了研究6种天然黄类化合物对β-LG抗原性和抗氧化活性的共价修饰的影响.
- 阐明β-LG和黄类药物之间的相互作用机制.
- 为了评估由黄化合物结合引起的结构变化.
主要方法:
- 乙-LG与六种天然黄酸的共价结合:EGCG,kaempferol,myricetin,phloretin,naringenin和Quercetin. 这六种类型的黄酸中含有
- 使用抑制率量化抗原性降低的量化.
- 评估由此产生的结合物的抗氧化能力.
- 使用光谱技术分析结构变化 (α-螺旋到β-片).
主要成果:
- 黄类合物显著降低了β-LG抗原性,EGCG表现出最高的抑制 (57.0%).
- 与原生β-LG相比,β-LG-类合物显示出增强的抗氧化能力.
- 随着黄类合,观察到显著的结构变化,包括从α-螺旋转移到β-螺旋转移.
结论:
- 用黄类药物对β-LG进行共价修饰是一种有效的降低其抗原性的策略.
- 黄化合物的结合改善了β-LG的抗氧化潜力.
- 由黄化合物结合引起的结构变化是导致功能性质的改变的原因,这表明改性β-LG的应用很有前途.
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