氧化降解潜力作为一种测定精液样本中氧化应激的方法
András Balló1,2,3, Péter Czétány2,3, Kinga Székvári Busznyákné1
1Pannon Reproduction Institute, 8300 Tapolca, Hungary.
International journal of molecular sciences
|August 12, 2023
概括
氧化应激 (OS) 影响男性不孕. 氧化还原潜力 (ORP) 测量可以检测到活性氧物种 (ROS),但需要正常化才能获得准确的结果,特别是变化的精子数量.
科学领域:
- 生殖医学与雄性病理学
- 氧化应激和男性生殖健康
背景情况:
- 不孕症影响全球15%的夫妇,氧化应激 (OS) 和反应性氧物种 (ROS) 与精液质量差有关.
- 目前缺乏安德罗学OS的诊断和治疗方案.
- 氧化降解潜力 (ORP) 测量提供了一个快速的方法来评估OS.
研究的目的:
- 为了验证ORP值和精子参数之间的相关性.
- 为了研究精子度对ORP测量的影响.
- 为了确定诱导的细胞内ROS形成是否反映在ORP值中.
主要方法:
- 细胞内ROS被诱导使用梅纳,过氧化,和三丁氧化.
- 用SCA Scope系统评估了精子的运动性和活力.
- 使用Mioxsys系统监测ORP变化.
主要成果:
- 在ORP,精子度,运动性,渐进性运动性和活力之间发现了显著的相关性,但只有当ORP被精子数正常化时.
- 人工ROS诱导降低了精子的活力和运动性,改变了ORP水平.
- 精液血显示出一种保护作用,其去除会增加精子对OS诱导剂和ORP水平的敏感性.
结论:
- ORP测量是一种可行的工具,用于检测细胞内ROS积累在andrology.
- 精子计数对ORP值的规范化对于准确的解释至关重要,因为极端度可能会误导.
- 需要进一步的研究来澄清临床实践中ORP测量的局限性.
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