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贝加尔湖和经过处理的废水中的RNA-Seq病毒分数
Sergey Potapov1, Anna Gorshkova1, Andrey Krasnopeev1
1Limnological Institute, Siberian Branch of the Russian Academy of Sciences, Ulan-Batorskaya 3, 664033 Irkutsk, Russia.
International journal of molecular sciences
|August 12, 2023
概括
污水处理厂不充分净化进入贝加尔湖的水,发现了大量的dsDNA病毒. 虽然RNA病毒在处理过的水中占主导地位,但在湖泊的海底区域没有检测到人类病原体.
科学领域:
- 环境病毒学 环境病毒学
- 微生物生态学 微生物生态学
- 分子生物学分子生物学
背景情况:
- 贝加尔湖面临着废水处理厂 (WWTP) 排放的潜在病毒学危险.
- 监测本土微生物对于维护贝加尔湖的生物多样性和人类健康至关重要.
研究的目的:
- 为了分析贝加尔湖和WWTP废水中的病毒群体.
- 确定对水生生物和人类的潜在病毒学风险.
主要方法:
- 病毒RNA和DNA的高通量测序.
- 对水样进行卫生和微生物学分析.
主要成果:
- dsDNA病毒 (Caudoviricetes) 总体上是最丰富的;ssRNA(+) 病毒 (Leviviricetes) 在WWTP废水中占主导地位.
- 多种RNA病毒,包括Nodaviridae和Dicistroviridae,在湖的海底海洋区域被发现.
- 湖水净化器的水净化不够,但在湖中没有发现人类致病病毒.
结论:
- 在WWTP污水引入显著的病毒载荷进入贝加尔湖.
- 虽然病毒的多样性很高,但目前的数据表明,湖中的海病毒对人类健康没有直接的风险.
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