在大麦中,干旱压力相关的基因表达的重编程涉及ABA相关基因的差异性希斯基因修饰
Charlotte Ost1, Hieu Xuan Cao2, Thuy Linh Nguyen1
1Institute of Biology, Martin Luther University Halle-Wittenberg, Weinbergweg 10, 06120 Halle, Germany.
International journal of molecular sciences
|August 12, 2023
概括
受干旱影响的大麦植物表现出显著的基因表达变化,特别是在酸 (ABA) 信号通路中. 像H3K9乙化这样的表观遗传修饰是敏感的干旱指标,揭示了参与植物应激反应的关键调节基因.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 植物激活复杂的基因表达程序,以生存环境压力,如干旱.
- 植物激素酸 (ABA) 是干旱反应中的关键信号分子.
- 在表观遗传和转录基因层面理解这些反应对于作物改进至关重要.
研究的目的:
- 为了研究大麦 (Hordeum vulgare) 的表观基因组和转录基因组级干旱反应.
- 在干旱压力期间通过 euchromatic 标记 (H3K4me3,H3K9ac) 修改的基因进行识别.
- 为了将表观遗传变化与基因表达和ABA信号通路相关联.
主要方法:
- 对大麦植物施加的干旱处理 (cv. 莫雷克斯) 在十天内.
- 测量生理参数:叶绿素含量和光系统II效率.
- 染色体免疫沉测序 (ChIP-Seq) 用于识别H3K4me3和H3K9ac的修饰.
- 不同基因表达分析.
主要成果:
- 干旱导致叶绿素含量和光系统II效率下降约10%.
- 观察到干旱诱导的基因 (例如, HvS40, HvA1).
- H3K9乙化 (2008年基因) 比H3K4三甲基化 (129基因) 更容易受到干旱的影响.
- 显著比例的表观遗传修饰和诱导基因参与了ABA信号传递,包括两个蛋白质酸酶2C (PP2C) 家族成员.
结论:
- H3K9ac是一种敏感的表观遗传标记物,用于大麦的干旱压力.
- 表观遗传重编程,特别是H3K9ac,在干旱引起的基因表达中起着关键作用.
- 包括PP2Cs在内的ABA信号通路基因在表观遗传和转录基因层面都受到干旱的显著调节.
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