短期和长期调节的HuD:一个分子开关通过叶酸介导?
Nicoletta Marchesi1, Pasquale Linciano2, Lucrezia Irene Maria Campagnoli1
1Department of Drug Sciences, Pharmacology Section, University of Pavia, 27100 Pavia, Italy.
International journal of molecular sciences
|August 12, 2023
概括
叶酸 (FA) 增加神经元中的RNA结合蛋白HuD和脑衍生神经营养因子 (BDNF),这表明FA在神经系统发育方面表观遗传调节HuD,并为神经系统疾病提供治疗潜力.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- RNA结合蛋白HuD对于神经系统基因调节至关重要,并与神经系统疾病有关.
- 大脑衍生神经营养因子 (BDNF) 对神经元发育,生存和功能至关重要,与神经元疾病有关.
研究的目的:
- 研究神经细胞中HuD,BDNF和叶酸 (FA) 之间的关系.
- 探索FA作为影响神经系统发育和HuD功能的环境因素的作用.
主要方法:
- 为HuD及其目标建立了一个交互网络.
- 进行了体外实验,使用神经元样的人类SH-SY5Y细胞治疗FA.
- 分析了HuD和BDNF转录和蛋白质水平的变化.
- 在FA暴露后研究了HuD甲基化.
主要成果:
- 在2小时和4小时后,FA暴露显著增加了SH-SY5Y细胞中的HuD和BDNF转录和蛋白质.
- 即使在经过一段时间的FA洗后,也观察到这些增加.
- 24小时FA暴露导致HuD和BDNF水平下降,与HuD甲基化相关.
- FA 证明了对HuD和BDNF的短期和长期调节效应.
结论:
- 叶酸通过神经元细胞中的HuD调节BDNF表达.
- 通过短期和长期机制,FA在神经系统中充当HuD的表观遗传调节器.
- 作为神经和精神疾病的治疗点,BDNF具有潜力.
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