在体外低氧状态下解开生理上老化的星体细胞的集体动力学
Elena V Mitroshina1, Mikhail I Krivonosov1, Alexander M Pakhomov1,2
1Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Avenue, 603022 Nizhny Novgorod, Russia.
International journal of molecular sciences
|August 12, 2023
概括
衰老的星体细胞表现出减少的信号和网络连接. 较老的星体细胞显示抗亡因子的补偿性增加,而缺氧会使年轻星体细胞的网络功能障碍恶化.
科学领域:
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
- 天星细胞生物学 天星细胞生物学
背景情况:
- 星球细胞对于大脑的平衡和神经元功能至关重要.
- 星细胞功能障碍与大脑衰老和神经退行有关.
- 细胞间信号传递是星体细胞的关键通信机制.
研究的目的:
- 为了研究衰老的天体细胞中的动态和网络架构.
- 为了在体外模拟衰老和缺氧对天体细胞通信的影响.
- 评估功能连接和分子反应的变化.
主要方法:
- 在不同发育阶段 (年轻人与老年人) 的初级小鼠皮质天体细胞培养物.
- 成像用于分析的动态和网络活动.
- 对细胞活动,事件频率和细胞间相关性的定量分析.
- 对Bcl-2和连接素的mRNA表达分析 43.
主要成果:
- 衰老的星体细胞显示细胞活动减少,事件频率和细胞间相关性.
- 功能性网络连接性在较老的星球细胞中下降,信号事件较少,速度较慢.
- 较老的星体细胞表现出增加的Bcl-2和连xin 43mRNA表达.
- 缺氧在年轻星球细胞中加剧了网络功能障碍,而不是老星球细胞.
结论:
- 随着年龄的增长,星细胞信号传递和网络连接性下降.
- 在衰老的星球细胞中,包括增加的Bcl-2和连xin43在内的补偿机制被激活.
- 缺氧对年轻人与老年天体细胞的网络动态产生不同的影响,突出了与年龄相关的脆弱性.
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