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克里斯普尔/Cas9:一个强大的策略来改善CAR-T细胞的持久性
Wei Wei1, Zhi-Nan Chen1, Ke Wang1
1National Translational Science Center for Molecular Medicine & Department of Cell Biology, Fourth Military Medical University, Xi'an 710032, China.
International journal of molecular sciences
|August 12, 2023
概括
化学抗原受体T (CAR-T) 细胞疗法对癌症有希望,但在持久性方面面临挑战. 克里斯普尔/Cas9技术增强了CAR-T细胞的增殖和寿命,改善了抗瘤潜力,以获得更好的癌症治疗结果.
科学领域:
- 免疫治疗是一种免疫疗法.
- 基因编辑技术 基因编辑技术
- 在瘤学瘤学.
背景情况:
- 化学抗原受体T (CAR-T) 细胞疗法是恶性瘤的新兴策略,在过去十年中效率有所提高.
- 尽管取得了进展,但CAR-T疗法面临着局限性,特别是在固体瘤中,并且由于CAR-T细胞扩张和持久性差,在血液恶性瘤中会发生复发.
研究的目的:
- 描述限制患者CAR-T细胞持久性的因素.
- 讨论CRISPR/Cas9技术在增强CAR-T细胞持久性和抗瘤功能的应用.
- 审查涉及CRISPR/Cas9工程CAR-T细胞用于癌症治疗的临床试验.
主要方法:
- 关于CAR-T细胞治疗局限性的当前文献的审查.
- 分析CRISPR/Cas9用于CAR-T细胞增强的应用 (例如,产生记忆表型,减少疲劳,查目标).
- 对CRISPR/Cas9修饰的CAR-T细胞进行的和已完成的临床试验的调查.
主要成果:
- 确定不良扩张和持久性是CAR-T细胞治疗的关键局限性.
- 突出了CRISPR/Cas9在改善CAR-T细胞增殖,持久性和抗瘤活性方面的潜力.
- 注意到越来越多的临床试验探索CRISPR/Cas9工程CAR-T细胞.
结论:
- 克里斯普尔/Cas9技术为克服CAR-T细胞持久性问题提供了一个有希望的方法.
- 通过基因编辑增强CAR-T细胞的持久性,可以改善各种癌症的治疗结果.
- 为了优化癌症治疗策略,需要对CRISPR/Cas9修饰的CAR-T细胞进行进一步的临床研究.
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