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在Kawasaki病和儿童多系统炎症综合征中的内皮细胞反应

Jihoon Kim^1,2, Chisato Shimizu³, Ming He⁴

¹Department of Biomedical Informatics, University of California, San Diego, CA 92093, USA.

International journal of molecular sciences

|August 12, 2023

概括

儿童多系统炎症综合征 (MIS-C) 和川崎病 (KD) 不同地影响内皮细胞 (EC). 在EC中,MIS-C对支持生存的途径进行上调,而KD影响EC恒温并促进过渡,这可能解释了各种心血管结果.

科学领域:

分子生物学分子生物学
免疫学免疫学免疫学
儿童心脏病学儿童心脏病学

背景情况:

川崎病 (KD) 和儿童多系统炎症综合征 (MIS-C) 是儿科炎症状况,症状重叠,但心血管结果不同.
内皮细胞 (ECs) 在心血管健康中起着至关重要的作用,在KD和MIS-C中可能会受到不同的影响,导致不同的临床轨迹.

研究的目的:

研究暴露于MIS-C患者血清的内皮细胞 (ECs) 的不同分子反应,与KD相比.
确定EC中的特定基因表达模式和途径,将MIS-C与KD区分开来,可能解释心血管并发症的差异.

主要方法:

进行RNA测序 (RNA-seq) 在培养的人类EC上进行,这些EC与MIS-C,KD和健康对照队伍的预处理血清进行化.
使用权重基因共同表达网络分析 (WGCNA) 和差异基因表达分析来确定关键的分子通路和转录.

主要成果:

在EC中,MIS-C血清诱导了TNFα/NFκB通路基因,支持生存的转录和与自相关的基因 (UBD,EBI3,SQSTM1) 的显著上调.
相反,与MIS-C.相比,KD血清与降低EC稳态和内皮-介质细胞过渡 (EndoMT) 相关的转录有关.
在MIS-C中,WGCNA确定了七个不同的基因模块,突出显示了改变的免疫反应,EC平衡和EndoMT.

结论:

当暴露于MIS-C与KD血清时,内皮细胞表现出明显的转录组形状,其特征在于MIS-C中增加了亲生存信号.
观察到的EC反应的差异,包括改变的平衡和EndoMT,可能是MIS-C和KD观察到的不同心血管后续的基础.

关键词:

在EndoMTMT中使用.川崎病是川崎病的一种疾病.这就是MIS-C的意义.通过NFκB路径.在RNA-seqqq.在WGCNA中,WGCNA是WGCNA.灭症 (apoptosis) 是一种死亡的过程.自自是自的过程.细胞内皮细胞的内皮细胞.网络分析网络分析

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