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相关概念视频

Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

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A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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相关实验视频

Updated: Jul 19, 2025

Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases
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Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases

Published on: March 11, 2016

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主要与次要的升高在底部自化自化.

Rait Parmann1, Stephen H Tsang1,2, Janet R Sparrow1,2

  • 1Departments of Ophthalmology, Columbia University, 635 W. 165th Street, New York, NY 10032, USA.

International journal of molecular sciences
|August 12, 2023
PubMed
概括

定量底部自光 (qAF) 在各种视网膜疾病中揭示了视网膜色素表皮 (RPE) 细胞中的高 bisretinoids. 一些疾病表现出直接的基因联系,而另一些疾病则表明二次光受体退化有助于增加素.

科学领域:

  • 眼科医生 眼科 眼科
  • 视网膜生物学 视网膜生物学
  • 分子遗传学 分子遗传学

背景情况:

  • 定量底部自光 (qAF) 测量了视网膜色素表皮 (RPE) 中的比斯雷类物质水平.
  • 升高的比斯雷类药物与各种视网膜疾病有关.
  • 了解qAF途径对于疾病管理至关重要.

研究的目的:

  • 审查七种视网膜疾病及其与 fundus自光增加的关联.
  • 探索导致RPE细胞中比斯雷类物质升高的病理生理路径.
  • 要区分qAF升高的初级和二级机制.

主要方法:

  • 关于七种视网膜疾病的文献综述.
  • 分析已知与bisretinoid代谢的遗传联系.
  • 对qAF升高的病理生理路径的调查.

主要成果:

  • 在ABCA4和RDH12疾病中,有直接的基因-bisretinoid升高路径.
  • 在PRPH2/RDS,RP,CSC,AZOOR和CERKL疾病中,高qAF呈现,但没有明确的bisretinoid通路.
  • 提出了qAF升高的双重机制:主要 (基因突变) 和次要 (光受体退化).
关键词:
基金斯的自光自体.光学连贯性断层扫描技术定量基金的自化自化.视网膜 视网膜 视网膜 是一个短波长的基底自光自发光.

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Author Spotlight: Understanding Age-Related Macular Degeneration Pathophysiology with QAF Workflow

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相关实验视频

Last Updated: Jul 19, 2025

Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases
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Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases

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Author Spotlight: Understanding Age-Related Macular Degeneration Pathophysiology with QAF Workflow
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Author Spotlight: Understanding Age-Related Macular Degeneration Pathophysiology with QAF Workflow

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Author Spotlight: Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration
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Author Spotlight: Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration

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结论:

  • 在ABCA4和RDH12中发生的基因突变直接增加了Bisretinoid水平.
  • 由于光受体损伤,其他视网膜疾病可能会出现二次qAF增加.
  • 需要进一步的研究来阐明各种视网膜退化中的二次qAF途径.