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索尔提林在关节炎依赖的软骨结石化中受到抑制,并与细胞衰老有关

Elisabeth Richter¹, Christoph H Lohmann¹, Francesco Dell'Accio²

¹Department of Orthopaedic Surgery, Otto von Guericke University Magdeburg, 39120 Magdeburg, Germany.

International journal of molecular sciences

|August 12, 2023

概括

骨关节炎涉及软骨化,与细胞衰老有关. 这项研究发现,随着化和衰老,sortilin和性酸酶 (ALP) 的增加,表明它们在骨关节炎发展中的作用.

科学领域:

生物化学生物化学
细胞生物学细胞生物学
整形外科整形外科整形外科

背景情况:

骨关节炎 (OA) 是一种退行性关节疾病,其特征是软骨化,疼痛和残疾.
在OA中软骨化与酸盐路径失衡和细胞衰老有关.
索尔提林是一种跨膜蛋白,通过性酸酶 (ALP) 囊泡参与血管化.

研究的目的:

调查索尔提林和细胞衰老在骨关节炎相关的软骨结石化中的潜在作用.

主要方法:

使用免疫组织化学和RT-PCR对人类和小鼠骨关节炎软骨的分析.
在体外研究中,使用使用骨质生媒介或米托米辛C治疗的人类红细胞来诱导化和衰老.
索尔提林和ALP的西部涂抹,ALP活性测定和基因表达分析.

主要成果:

在患有骨关节炎的人类和小鼠膝关节的软骨细胞中增加了sortilin和p16 (衰老标记物) 的表达.
在试验室中诱导软骨化过程中,Sortilin蛋白和基因表达的升级.
晚些时候在化过程中观察到ALP水平和活性升高;衰老的红细胞显示索尔提林和ALP基因表达增加.

结论:

索尔提林和ALP在骨关节炎的软骨化过程中被上调,与状细胞衰老相关.
细胞衰老似乎会诱导索尔提林的表达,这表明有助于OA病变的机制.
索尔提林和ALP可能在与骨关节炎相关的软骨化发展中发挥重要作用.

关键词:

化的化过程软骨软骨软骨是一种冠状腺细胞 (chondrocytes) 是一种体内细胞.骨关节炎是一种关节炎.衰老是一种老化.这种类型的LINK.

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