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在实验性透析条件下,生物工程管有效清除尿素毒素
João Faria1, Sabbir Ahmed1, Dimitrios Stamatialis2
1Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, 3584 CG Utrecht, The Netherlands.
International journal of molecular sciences
|August 12, 2023
概括
透析液对生物人工细胞是安全的,有效地去除与蛋白质结合的尿素毒素. 这一发现支持为末期病患者开发生物人工脏.
科学领域:
- 生物医学工程 生物医学工程
- 腎臟病學 (nephrology) 是一種醫學專業.
- 细胞生物学 细胞生物学
背景情况:
- 末期病 (ESKD) 患者会积累与蛋白质结合的尿素毒素 (PBUT),导致并发症.
- 目前的透析方法无法充分去除这些毒素.
- 使用近接管上皮细胞 (PTECs) 的生物人工脏 (BAKs) 显示出 PBUT 移除的希望,但透析液 (DF) 的细胞相容性尚不清楚.
研究的目的:
- 评估透析液 (DF) 与人类条件永生PTECs (ciPTECs) 的细胞相容性.
- 评估DF对ciPTEC可行性,单层完整性和PBUT清除的影响.
- 确定DF在支持BAK功能方面的潜力.
主要方法:
- 在各种支上培养的ciPTEC:井板,Transwell®插件和空心纤维膜 (HFMs).
- 评估了DF暴露后的细胞活力标记物和单层完整性.
- 从 perfusion 液和尿液血中测量了 anionic PBUT 的清除.
主要成果:
- 暴露于DF并没有损害ciPTEC的生存能力或单层完整性.
- ciPTECs证明了七种阳离子PBUT的有效清除.
- 通过ciPTECs,白蛋白的存在增强了PBUT清除.
结论:
- 透析液与PTEC细胞相容.透析液与PTEC细胞相容.
- DF不会损害ciPTEC的功能,支持BAK的发展.
- 这些发现推动了生物人工的潜在临床应用.
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