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在BrAHL基因的非生物压力下的全基因组识别和表达分析在Brassica rapa中的基因

Xiaoyu Zhang¹, Jiali Li¹, Yunyun Cao¹

¹College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an 271018, China.

International journal of molecular sciences

|August 12, 2023

概括

这项研究确定了Brassica rapa中的42个AT-hook motif核局部化 (AHL) 基因,揭示了它们在植物生长和应激反应中的作用. 关键的BRAHL基因在干旱,寒冷和重金属压力下显示出多样化的表达.

科学领域:

植物分子生物学植物分子生物学
基因组学就是基因组学.
转录因子转录因子

背景情况:

核局部化 (AHL) 基因家族对植物生长,发育和应激耐受性至关重要.
在Brassica rapa (B. rapa) 中,AHL基因家族的特定功能尚不清楚.

研究的目的:

为了识别和描述B. rapa.中的AHL基因家族.
研究BRAHL基因的进化关系,基因组分布和潜在功能,特别是对环境压力的反应.

主要方法:

在B. rapa. 中对AHL基因的全基因组识别和家族遗传学分析.
在各种压力条件下分析 cis-regulatory 元素和基因表达特征.
预测AHL基因的蛋白质-蛋白质相互作用网络.

主要成果:

42个AHL基因 (BrAHLs) 被识别并映射到B. rapa的9个染色体中,有基因重复的证据.
遗传学分析显示,BrAHL家族内有两个主要分类.
调节性元素分析表明它们在压力 (奥斯摩斯,冷,重金属),激素 (酸) 和光反应中的作用.
表达特征分析显示,在干旱,寒冷和压力下,特定的BRAHL基因 (BrAHL16,BRAHL02,BRAHL24) 的广泛组织表达和差异调节.
蛋白质-蛋白质相互作用网络预测表明在压力条件下的潜在相互作用.

结论:

BrAHL基因家族通过基因重复扩大,并在B. rapa对各种环境压力的反应中发挥重要作用.
特定的BRAHL基因在压力下表现出不同的表达模式,突出显示了它们的专业功能.
对BrAHL基因功能和相互作用的进一步研究是有必要的,以了解植物应激适应机制.

关键词:

在 AHL AHL 中.黄铜 (Brassica rapa) 是一种葡萄.无生物压力是无生物压力.生物信息学是一种生物信息学.表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式

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