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揭示了从内生菌Penicillium中提取的多糖的结构特征和生物活性
Kumar Vishven Naveen1, Anbazhagan Sathiyaseelan1, Sumana Mandal2
1Department of Bio-Health Convergence, Kangwon National University, Chuncheon 24341, Republic of Korea.
Molecules (Basel, Switzerland)
|August 12, 2023
概括
来自 Penicillium radiatolobatum 的多糖体具有强大的抗氧化和抗癌特性. 细胞内多糖-2 (IPS-2) 证明对前列腺癌细胞具有显著的有效性,而不会损害健康细胞.
科学领域:
- 菌类学 菌类学是指菌类学.
- 生物化学 生物化学
- 药理学 药理学是指药理学的学科.
背景情况:
- 菌多糖因其显著的生物活性而闻名.
- 内生真菌,如Penicillium radiatolobatum,是新型生物活性化合物的来源.
研究的目的:
- 从Penicillium radiatolobatum中提取和表征细胞内多糖体 (IPS-1和IPS-2).
- 为了比较IPS-1和IPS-2的物理化学和生物活性特性.
- 评估这些真菌多糖的抗氧化和抗癌潜力.
主要方法:
- 从Penicillium radiatolobatum中提取细胞内多糖 (IPS-1,IPS-2) 的方法.
- 单糖化合物组成分析,FT-IR,1H NMR和13C NMR用于结构阐明.
- 在体外测定抗氧化活性 (ABTS+,DPPH,铁的降解功率) 和细胞毒性对前列腺癌 (PC-3) 和非癌细胞 (HEK-293,NIH3T3).
主要成果:
- IPS-2结构的特征是 β-(1→4) -D-银糖和 β-(1→4) -D-葡萄糖主链与 β-(1→6) -D-曼诺斯分支.
- 与IPS-1相比,IPS-2表现出更高的抗氧化活性.
- IPS-2通过诱导细胞灭亡显著抑制前列腺癌细胞活力,在非癌细胞中没有观察到毒性.
结论:
- 尼 (Penicillium radiatolobatum) 是一种有价值的生物活性多糖的来源.
- IPS-2具有显著的抗氧化和选择性抗癌特性,需要进一步研究治疗应用.
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